Description

4-Methylumbelliferyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt is a high-purity, water-soluble salt form of the widely used fluorogenic substrate 4-methylumbelliferyl phosphate (4-MUP). This compound matters as a critical reagent in enzymatic assays, where its hydrolysis by phosphatases releases the highly fluorescent 4-methylumbelliferone, enabling sensitive detection and quantification. It is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors who require reliable, high-performance substrates for enzyme-linked assays and diagnostic kits.

Application

• Clinical Diagnostics: As a key substrate in automated clinical chemistry analyzers for measuring alkaline phosphatase (ALP) and acid phosphatase (ACP) activity, aiding in the diagnosis of liver, bone, and prostate disorders.
• Enzyme-Linked Immunosorbent Assay (ELISA): Used in fluorogenic ELISA protocols for the detection of antibodies, antigens, or hormones with high sensitivity and a broad dynamic range.
• Molecular Biology & Biochemistry Research: Employed in kinetic studies, enzyme characterization, and high-throughput screening (HTS) assays to monitor phosphatase activity.
• Diagnostic Kit Manufacturing: A vital raw material for the formulation of commercial diagnostic test kits and reagents for clinical and veterinary use.
• Microbiology: Used in fluorogenic culture media and assays for the detection and identification of specific microorganisms based on their enzymatic profiles.
• Cell Biology: Applied in histochemistry and cytochemistry for the localization of phosphatase activity within tissues and cells.
• Food & Environmental Testing: Utilized in assays to detect bacterial contamination or to monitor phosphatase activity as an indicator of pasteurization efficacy in dairy products.

Basic Information

Product Name	4-Methylumbelliferyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt
CAS No.	107475-10-5
Molecular Formula	C19H32N2O10P
Molecular Weight	464.44 g/mol
Synonyms	4-MUP, Bis(AMP) Salt; 4-Methylumbelliferyl Phosphate, AMP Salt; 4-Methylumbelliferyl Dihydrogen Phosphate, Compd. with 2-Amino-2-methyl-1,3-propanediol (2:1); 7-Hydroxy-4-methylcoumarin phosphate, 2-Amino-2-methyl-1,3-propanediol salt; Fluorogenic phosphatase substrate; 4-Methylumbelliferyl phosphate dicyclohexylammonium salt (alternative salt form); 4-MU-P; 4-Methylumbelliferyl phosphate, 2-amino-2-methyl-1,3-propanediol
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Phosphate, Bis(AMP) Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure it meets the high-purity standards required for sensitive diagnostic and research applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for identity, purity (HPLC), fluorescence performance, and impurity profiles. We support compliance with relevant pharmacopeial guidelines and cGMP principles for pharmaceutical raw materials.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, elevated temperature, or light may lead to decomposition and reduced performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Fluorescence Intensity	Meets performance criteria
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solubility (in Water)	Clear, colorless solution at 1 mg/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.