Description

n-Succinimidyl-3-(2-Pyridyldithio)Butyrate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a sulfhydryl-reactive pyridyldithiol group. This compound matters for its critical role in the site-specific conjugation of biomolecules, enabling the creation of stable, well-defined antibody-drug conjugates (ADCs), protein-protein conjugates, and other advanced bioconjugates. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require high-purity, reliable linkers for next-generation therapeutic and diagnostic platforms.

Application

• Antibody-Drug Conjugate (ADC) Development: A key reagent for linking cytotoxic payloads to monoclonal antibodies via lysine residues.
• Protein Labeling & Modification: For introducing fluorescent dyes, enzymes, or other probes onto proteins at primary amines.
• Immobilization Chemistry: Creating affinity chromatography supports by coupling ligands to agarose or other matrices.
• Peptide & Oligonucleotide Conjugation: Building complex biomolecular constructs for research and therapeutic applications.
• Diagnostic Assay Development: Used in the manufacture of enzyme-linked immunosorbent assays (ELISAs) and other immunoassays.
• Biomaterial & Surface Functionalization: Modifying surfaces with bioactive molecules for cell culture, biosensors, and medical devices.

Basic Information

Product Name	n-Succinimidyl-3-(2-Pyridyldithio)Butyrate
CAS No.	107348-47-0
Molecular Formula	C15H15N3O4S2
Molecular Weight	365.43 g/mol
Synonyms	SPDB; N-Succinimidyl 3-((2-pyridyldithio)butyrate); 3-((2-Pyridyldithio)butyric acid N-hydroxysuccinimide ester; SPDB Crosslinker; SPDB NHS Ester; SPDB Reagent; N-Succinimidyl 3-(2-pyridyldithio)butanoate; 3-(2-Pyridyldithio)butyric Acid Succinimidyl Ester
EINECS	Contact for details

Quality Control

Our n-Succinimidyl-3-(2-Pyridyldithio)Butyrate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and moisture analysis. We provide comprehensive Certificates of Analysis (COA) with each shipment to ensure traceability and compliance with your research or cGMP-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.