Description

p-Nitrophenyl β-D-Cellotrioside is a specialized chromogenic substrate designed for the sensitive and specific detection of cellulase enzyme activity. This compound matters as a critical research tool for quantifying endo-1,4-β-glucanase activity, enabling precise biochemical analysis in enzymatic studies. It is primarily needed by researchers and quality control laboratories in the biofuel, pulp & paper, textile, and animal feed industries for enzyme characterization and development.

Application

• Cellulase Enzyme Assays: Primary substrate for the colorimetric determination of endo-1,4-β-glucanase (cellulase) activity.
• Biomass Conversion Research: Used in studies to evaluate and optimize enzymatic hydrolysis of cellulose for biofuel production.
• Enzyme Kinetics & Characterization: Essential for studying enzyme mechanisms, inhibition, and determining kinetic parameters (Km, Vmax).
• Industrial Process Monitoring: Employed in quality control to monitor cellulase activity in commercial enzyme preparations for textiles, detergents, and paper processing.
• Microbiological & Agricultural Research: Used to assay cellulase production by microorganisms, fungi, or in studies related to plant cell wall degradation.
• Diagnostic Reagent Development: Serves as a key component in developing test kits for cellulase activity in various samples.

Basic Information

Product Name	p-Nitrophenyl β-D-Cellotrioside
CAS No.	106927-48-4
Molecular Formula	C24H33NO17
Molecular Weight	607.52 g/mol
Synonyms	4-Nitrophenyl β-D-cellotrioside; pNP-Cellotrioside; 4-Nitrophenyl β-cellotrioside; p-Nitrophenyl-β-cellotrioside; β-4-Nitrophenyl cellotrioside; Cellotrioside p-nitrophenyl β-D-; 1,4-β-Cellotriosyl p-nitrophenyl β-D-glucoside; PNPC3
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-Cellotrioside is produced under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive enzymatic research. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.