Description

Maleimidobutyrylbiocytin CAS NO 106873-92-1 is a specialized heterobifunctional crosslinking reagent designed for bioconjugation, combining a maleimide group for thiol-specific coupling with a biocytin moiety for avidin/biotin-based detection or purification. This compound is critical for researchers and developers requiring precise, stable labeling of proteins, peptides, and other biomolecules without disrupting their native structure or function. It is an essential tool in life sciences R&D, particularly for applications in proteomics, antibody-drug conjugate (ADC) development, and advanced diagnostic assay design.

Application

• Bioconjugation and Protein Labeling: Site-specific covalent attachment of biotin tags to cysteine residues on antibodies, enzymes, and other proteins for detection and immobilization.
• Proteomics Research: Used in pull-down assays and affinity purification to isolate and study protein-protein interactions and post-translational modifications.
• Antibody-Drug Conjugate (ADC) Development: Serves as a linker-payload component for constructing stable, targeted therapeutics by conjugating cytotoxic agents to monoclonal antibodies.
• Diagnostic Assay Development: Enables the creation of highly sensitive ELISA, lateral flow, and other immunoassay formats by biotinylating detection antibodies or antigens.
• Cell Surface Engineering: Labeling of cell surface thiol groups for tracking, sorting, or targeting studies in flow cytometry and microscopy.
• Nanoparticle Functionalization: Conjugation of targeting ligands or reporter molecules to nanocarriers for drug delivery and imaging applications.
• Biomaterial Modification: Introduction of biotin handles onto hydrogels, chips, or other surfaces to create customizable platforms for biosensing and tissue engineering.

Basic Information

Product Name	Maleimidobutyrylbiocytin
CAS No.	106873-92-1
Molecular Formula	C₂₄H₄₀N₆O₇S
Molecular Weight	556.68 g/mol
Synonyms	N-(4-Maleimidobutyryl)biocytin; Maleimidobutyryl Biocytin; Biocytin Maleimide; Biocytin-4-maleimide; N-ε-Biotinyl-L-lysine Maleimide; N-(3-Maleimidopropionyl)biocytin (common variant); MBBC; Biotin-PEG4-Maleimide (related compound); N-(Biotinoyl)-N'-(4-maleimidobutyryl)-L-lysine
EINECS	Contact for details

Quality Control

Our Maleimidobutyrylbiocytin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment. We support development under cGMP guidelines where required.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.