Description

n-Sulfosuccinimidyl-(4-Iodoacetyl)-Aminobenzoate CAS NO 106145-13-5 is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a thiol-reactive iodoacetyl group. This compound is engineered for the efficient and site-specific conjugation of biomolecules, enabling the creation of stable thioether bonds. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and protein labeling, where precise and controlled linkage is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein-Protein Crosslinking: To study protein interactions or create stable protein complexes for analytical or therapeutic purposes.
• Peptide & Protein Immobilization: For covalently attaching peptides or proteins to solid supports (e.g., sensor chips, beads, plates) through free thiol groups.
• Fluorescent & Biotin Labeling: To introduce fluorescent dyes or biotin tags onto proteins, antibodies, or other amine-containing molecules via a thiol-reactive handle.
• Enzyme Modification: For the targeted modification of enzymes to alter activity, stability, or to facilitate immobilization.
• Diagnostic Reagent Synthesis: In the development of immunoassay components and other diagnostic tools requiring stable biomolecular conjugates.

Basic Information

Product Name	n-Sulfosuccinimidyl-(4-Iodoacetyl)-Aminobenzoate
CAS No.	106145-13-5
Molecular Formula	C13H10IN2O7S
Molecular Weight	489.20 g/mol
Synonyms	SIAB; N-Succinimidyl 4-(Iodoacetamido)benzoate; 4-(N-Iodoacetamido)benzoic Acid N-Hydroxysuccinimide Ester; 4-(Iodoacetamido)benzoic Acid Succinimidyl Ester; N-Succinimidyl (4-Iodoacetyl)Aminobenzoate; 4-((Iodoacetyl)amino)benzoic Acid N-Succinimidyl Ester; N-Sulfosuccinimidyl 4-(Iodoacetamido)benzoate; Heterobifunctional Crosslinker SIAB
EINECS	Contact for details

Quality Control

Our n-Sulfosuccinimidyl-(4-Iodoacetyl)-Aminobenzoate is manufactured under strict quality protocols suitable for research and bioconjugation applications. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance. Certificates of Analysis (COA) detailing purity, identity, and functional suitability are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.