Description

2',7'-Dichlorodihydrofluorescein is a key fluorogenic substrate and intermediate in the synthesis of reactive oxygen species (ROS) detection probes. Its primary value lies in its conversion to highly fluorescent dichlorofluorescein upon oxidation, making it a critical tool for measuring oxidative stress in biological systems. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic reagent industries who require reliable, high-purity biochemical tools.

Application

• Core Biochemical Assay Reagent: As the direct precursor to 2',7'-Dichlorofluorescein (DCFH), it is the fundamental substrate in the DCFH-DA assay for detecting and quantifying intracellular reactive oxygen species (ROS) like hydrogen peroxide and peroxynitrite.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to evaluate the antioxidant properties or pro-oxidant effects of novel drug candidates in cell-based models.
• Toxicology Studies: Employed to assess oxidative stress induced by environmental toxins, nanomaterials, or industrial chemicals in various cell lines and tissues.
• Diagnostic Kit Manufacturing: Serves as a key raw material for producing commercial assay kits used in research laboratories, clinical settings, and contract research organizations (CROs).
• Neuroscience and Aging Research: Applied in studies investigating the role of oxidative damage in neurodegenerative diseases, aging processes, and metabolic disorders.
• Food & Nutraceutical Analysis: Utilized in bioassays to measure the antioxidant capacity of natural extracts, vitamins, and functional food ingredients.

Basic Information

Product Name	2',7'-Dichlorodihydrofluorescein
CAS No.	106070-31-9
Molecular Formula	C20H12Cl2O5
Molecular Weight	403.21 g/mol
Synonyms	DCFH; 2',7'-Dichlorofluorescin; Dihydro-2',7'-dichlorofluorescein; 2',7'-Dichloro-3',6'-dihydroxyfluoran; 2,7-Dichlorodihydrofluorescein; 3',6'-Dihydroxy-2',7'-dichlorospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; H2DCF; 2',7'-Dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
EINECS	Contact for details

Quality Control

Our 2',7'-Dichlorodihydrofluorescein is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. We provide comprehensive analytical data, including HPLC purity, identity confirmation (IR, NMR), and tests for residual solvents and moisture. Certificates of Analysis (COA) detailing all specifications are available for every batch upon request, supporting your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorogenic Activity	Functional in standard DCF assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.