Description

Nbd-C 12 -Hpc [2-(12-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Amino)Dodecanoyl-1-Hexadecanoyl-Sn-Glycero-3-Phosphocholine] is a high-purity, fluorescently labeled phospholipid derivative, specifically designed for advanced research and diagnostic applications. This compound is critical for studying membrane dynamics, lipid trafficking, and cellular processes due to its specific incorporation into lipid bilayers and strong fluorescence emission. It is an essential reagent for researchers and developers in biophysics, cell biology, and diagnostic assay development who require reliable, well-characterized probes for sensitive detection and imaging.

Application

• Fluorescent Cell Membrane Labeling: For real-time visualization and tracking of membrane fluidity, fusion, and trafficking in live-cell imaging.
• Liposome & Model Membrane Studies: As a key component in the formulation of fluorescently tagged liposomes for drug delivery research and membrane biophysics.
• Biomolecular Interaction Assays: Used in fluorescence resonance energy transfer (FRET) or fluorescence polarization assays to study protein-lipid interactions.
• Diagnostic Kit Development: Serves as a critical fluorescent probe in the development of sensitive in-vitro diagnostic (IVD) tests and biosensors.
• Apoptosis & Cell Signaling Research: To investigate processes like phosphatidylserine externalization during apoptosis.
• High-Content Screening (HCS): Utilized in automated imaging platforms for phenotypic screening in drug discovery.

Basic Information

Product Name	Nbd-C 12 -Hpc [2-(12-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Amino)Dodecanoyl-1-Hexadecanoyl-Sn-Glycero-3-Phosphocholine]
CAS No.	105539-26-2
Molecular Formula	C₄₆H₈₁N₄O₁₀P
Molecular Weight	881.13 g/mol
Synonyms	NBD-C12-HPC; 1-Hexadecanoyl-2-[12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphocholine; NBD-PC (C12:0); Fluorescent Phosphatidylcholine; N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-12-aminododecanoyl-HPC; NBD labeled Phosphatidylcholine; 2-(NBD-dodecanoyl)-1-palmitoyl-sn-glycero-3-phosphocholine; NBD C12 Phosphocholine
EINECS	Contact for details

Quality Control

Every batch of our fluorescent phospholipids is produced and analyzed under strict quality protocols to ensure high purity, batch-to-batch consistency, and optimal performance in sensitive applications. Our quality assurance includes comprehensive analytical testing via HPLC and spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and fluorescence properties.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is both light-sensitive and hygroscopic. For long-term stability, store under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in chloroform, DMSO, ethanol
Extinction Coefficient (ε)	~21,000 cm⁻¹M⁻¹ (in methanol, ~470 nm)
Fluorescence Emission	~530 nm (in lipid membranes, excitation ~470 nm)
Water Content (KF)	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.