Description

5-Azidouridine-5'-Triphosphate is a chemically modified nucleotide triphosphate analog, specifically designed for advanced biochemical research and diagnostic applications. This compound is valued for its unique azido functional group, which enables efficient click chemistry reactions for labeling and crosslinking studies. It is primarily utilized by researchers and developers in the fields of molecular biology, enzymology, and next-generation sequencing (NGS) probe development.

Application

• Click Chemistry Labeling: Acts as a key substrate for bioorthogonal conjugation reactions to label nucleic acids with fluorophores, biotin, or other probes.
• Enzymatic Studies: Used as a substrate or inhibitor to study the activity and mechanism of DNA/RNA polymerases, reverse transcriptases, and other nucleotide-processing enzymes.
• Next-Generation Sequencing (NGS): Employed in the synthesis of specialized probes or for the incorporation of unique tags during library preparation.
• Crosslinking & Structural Biology: Facilitates photoaffinity labeling and crosslinking experiments to investigate nucleic acid-protein interactions and complex structures.
• Diagnostic Assay Development: Serves as a critical component in the development of sensitive molecular diagnostic kits and assays.
• Antiviral Research: Investigated for its potential as a modified nucleoside analog in antiviral drug discovery programs.

Basic Information

Product Name	5-Azidouridine-5'-Triphosphate
CAS No.	105518-68-1
Molecular Formula	C9H13N6O14P3
Molecular Weight	546.11 g/mol
Synonyms	5-Azido-UTP; 5-N3-UTP; Azido-UTP; 5-Azidouridine triphosphate; 1-((2R,3S,4R,5R)-5-(Azido)-3,4-dihydroxyoxolan-2-yl)-5-azidopyrimidine-2,4-dione 5'-(trihydrogen triphosphate); UTP-5-N3; 5-Azido-2'-deoxyuridine-5'-triphosphate (common misnomer, verify structure); 5-N3-Uridine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our 5-Azidouridine-5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. A Certificate of Analysis (COA) detailing purity, identity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5%
Biological Activity (Enzymatic Incorporation)	Functional as substrate (Contact for data)
Solubility	Soluble in water, TE buffer, or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.