Description

Z-Val-Leu-Arg-4Mβna · Hcl is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound is critical for researchers and quality control laboratories requiring precise enzymatic assays. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic kit manufacturing for studying serine proteases like thrombin and related enzymes.

Application

• Biochemical Research: As a specific fluorogenic substrate for kinetic studies and inhibition assays of serine proteases, including thrombin, trypsin, and plasmin.
• Pharmaceutical Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic applications.
• Diagnostic Reagents: A key component in the formulation of clinical diagnostic kits for measuring coagulation factors and other protease-related biomarkers.
• Enzyme Characterization: For determining enzyme specificity, catalytic efficiency (kcat/Km), and optimal reaction conditions in vitro.
• Quality Control: Employed in the QC testing of biopharmaceuticals, such as recombinant proteins, to ensure the absence of residual protease activity.

Basic Information

Product Name	Z-Val-Leu-Arg-4Mβna · Hcl
CAS No.	105317-35-9
Molecular Formula	C33H46N8O7 · HCl
Molecular Weight	703.23 g/mol (free base)
Synonyms	Benzyloxycarbonyl-Val-Leu-Arg-4-Methoxy-β-naphthylamide Hydrochloride; Z-Val-Leu-Arg-MNA · HCl; Boc-Val-Leu-Arg-MCA (related analog); Thrombin Substrate I (fluorogenic); Protease Fluorogenic Substrate; N-Benzyloxycarbonyl-L-valyl-N5-(Nα-benzyloxycarbonyl-L-arginyl)-L-leucine 4-methoxy-β-naphthylamide hydrochloride
EINECS	Contact for details

Quality Control

Our Z-Val-Leu-Arg-4Mβna · Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via mass spectrometry (MS) and infrared (IR) spectroscopy. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided to ensure compliance with research and in-vitro diagnostic (IVD) grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Identification (IR)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.