Description

Biotinyl-n-Hydroxysulfosuccinimide Ester is a high-purity, water-soluble chemical reagent designed for efficient bioconjugation. This compound is critical for introducing biotin tags onto primary amines in proteins, peptides, and other biomolecules under mild aqueous conditions. It is an essential tool for researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors who require reliable and specific labeling for detection, purification, and assay development.

Application

• Protein and Antibody Biotinylation: Primary reagent for labeling lysine residues to create biotinylated probes for immunoassays (ELISA, Western Blot) and diagnostic kits.
• Biomolecule Immobilization: Facilitating the site-directed attachment of proteins, peptides, or nucleic acids to streptavidin/avidin-coated surfaces for biosensors, microarrays, and affinity chromatography.
• Drug Conjugate Development: Used in the research and development of biotin-drug conjugates for targeted delivery systems and biopharmaceuticals.
• Cell Surface Labeling: For tagging cell surface proteins with biotin for subsequent isolation, tracking, or analysis via flow cytometry and microscopy.
• Pull-Down Assays: Key component in preparing bait molecules for streptavidin-bead based pull-down assays to study protein-protein interactions.
• In Vitro Diagnostic (IVD) Reagent Manufacturing: Serves as a critical raw material in the production of conjugated detection reagents for clinical diagnostics.

Basic Information

Product Name	Biotinyl-n-Hydroxysulfosuccinimide Ester
CAS No.	105248-43-9
Molecular Formula	C14H19N3O8S2
Molecular Weight	421.45 g/mol
Synonyms	Biotin Sulfo-NHS Ester; Biotinamidocaproyl-6-aminohexanoic Acid N-Hydroxysulfosuccinimide Ester; Biotin-XX SSE; Sulfo-NHS-LC-Biotin; Biotin-(AC5)2-Sulfo-Osu; N-Succinimidyl-6-(biotinamido)hexanoate Sulfonate; Biotin Long Chain Sulfosuccinimidyl Ester; EZ-Link Sulfo-NHS-LC-Biotin
EINECS	Contact for details

Quality Control

Our Biotinyl-n-Hydroxysulfosuccinimide Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.