Description

β-Secretase Substrate Iv, Fluorogenic is a specialized, fluorogenic peptide substrate designed for the sensitive detection and quantification of β-Secretase 1 (BACE1) enzyme activity. This compound is critical for high-throughput screening (HTS) and biochemical research focused on Alzheimer's disease and related neurological disorders. It is an essential tool for pharmaceutical R&D teams, academic researchers, and diagnostic developers working on BACE1 inhibitor discovery and enzymatic assay development.

Application

• Primary Screening Assays: Used in fluorogenic high-throughput screening (HTS) platforms to identify and characterize novel BACE1 inhibitors for Alzheimer's disease drug discovery.
• Enzyme Kinetic Studies: Enables the determination of kinetic parameters (Km, Vmax, kcat) for BACE1, facilitating detailed mechanistic studies of enzyme function and inhibition.
• Biochemical Research: Serves as a key reagent in basic research to study the proteolytic processing of the amyloid precursor protein (APP) and the role of BACE1 in amyloid-beta peptide production.
• Diagnostic Development: Employed in the development of in vitro diagnostic (IVD) assays to measure BACE1 activity in biological samples for research purposes.
• Compound Profiling & Selectivity Testing: Utilized to assess the potency and selectivity of potential therapeutic compounds against BACE1 versus other aspartyl proteases.

Basic Information

Product Name	β-Secretase Substrate Iv, Fluorogenic
CAS No.	104903-79-9
Molecular Formula	C41H57FN8O15
Molecular Weight	920.95 g/mol
Synonyms	BACE1 Substrate IV, Fluorogenic; Fluorogenic BACE1 Substrate; (7-Methoxycoumarin-4-yl)acetyl-SEVNLDAEFK(Dnp)-RR-NH2; Mca-SEVNLDAEFK(Dnp)-RR-NH2; β-Secretase 1 Fluorogenic Peptide Substrate; APP Substrate for BACE1; Alzheimer's Disease Research Substrate
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure batch-to-batch consistency and performance in sensitive enzymatic assays. Each lot is characterized by multiple analytical techniques to confirm identity, purity, and functional activity. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as purity (by HPLC), peptide content, and functional performance in a standardized BACE1 assay.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥95.0%
Peptide Content	≥70% (by weight)
Water Content (KF)	≤5.0%
Functional Activity	Conforms to reference standard in BACE1 assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.