Description

4-Nitrophenyl-β-D-Melibioside is a chromogenic synthetic substrate specifically designed for enzymatic assays. This compound is of critical importance for the precise measurement of α-galactosidase activity in research and diagnostic applications. It is primarily needed by biochemical researchers, diagnostic kit manufacturers, and quality control laboratories in the pharmaceutical and food science industries.

Application

• Enzymatic Substrate for α-Galactosidase: Primary use as a chromogenic substrate for the spectrophotometric determination of α-galactosidase (melibiase) enzyme activity.
• Biochemical Research: Used in studies of enzyme kinetics, inhibition, and characterization of α-galactosidase from various biological sources.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for genetic disorders like Fabry disease, which involves α-galactosidase A deficiency.
• Food & Beverage Industry Analysis: Employed in assays to monitor enzyme activity in processes involving raffinose family oligosaccharides, such as in sugar beet processing or soy product manufacturing.
• Microbiology & Biotechnology: Useful for screening microbial cultures for α-galactosidase production or in genetic engineering studies where this enzyme is a reporter.
• Quality Control (QC) in Enzyme Production: Serves as a standard substrate for the QC testing of purified α-galactosidase enzyme batches.

Basic Information

Product Name	4-Nitrophenyl-β-D-Melibioside
CAS No.	104872-92-6
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	p-Nitrophenyl β-D-melibioside; 4-Nitrophenyl β-melibioside; PNPMel; PNP-α-D-Gal-(1→6)-α-D-Glc; 4-Nitrophenyl-6-O-α-D-galactopyranosyl-β-D-glucopyranoside; 6-O-α-D-Galactopyranosyl-β-D-glucopyranoside 4-nitrophenyl ester; Melibiose 4-nitrophenyl glycoside; α-Galactosidase substrate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-β-D-Melibioside is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency suitable for sensitive enzymatic assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzyme assays. We adhere to stringent quality management practices to meet the exacting requirements of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Enzymatic Activity	Suitable for α-galactosidase assays
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.