Description

Methoxycarbonyl-D-Nle-Gly-Arg-Pna is a specialized synthetic peptide derivative, primarily used as a chromogenic substrate for the study of serine proteases. Its value lies in its high specificity and sensitivity for enzymatic activity assays, enabling precise biochemical research and diagnostic development. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent industries who require reliable tools for enzyme kinetics and inhibitor screening.

Application

• Biochemical Research: As a chromogenic substrate for the kinetic analysis and characterization of serine proteases, including thrombin, trypsin, and related enzymes.
• Pharmaceutical Development: Used in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors for drug discovery programs.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring coagulation factors and other protease activities in blood samples.
• Enzyme Mechanism Studies: Employed to investigate substrate specificity, catalytic efficiency, and the mechanistic pathways of proteolytic enzymes.
• Quality Control in Biologics: Utilized in potency assays to ensure the consistency and activity of enzyme-based therapeutic products during manufacturing.

Basic Information

Product Name	Methoxycarbonyl-D-Nle-Gly-Arg-Pna
CAS No.	104186-68-7
Molecular Formula	C24H38N8O7
Molecular Weight	550.61 g/mol
Synonyms	MOC-D-Nle-Gly-Arg-pNA; Methoxycarbonyl-D-norleucyl-glycyl-arginyl-4-nitroanilide; D-Nle-Gly-Arg-pNA, MOC-; Chromogenic Substrate for Serine Proteases; Thrombin Substrate MOC-D-Nle-Gly-Arg-pNA; Boc-D-Nle-Gly-Arg-pNA (common alternate); Tosyl-Gly-Pro-Arg-pNA analog; Protease Substrate 104186-68-7
EINECS	Contact for details

Quality Control

Our Methoxycarbonyl-D-Nle-Gly-Arg-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and functional activity validation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all specifications and test results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	≥ 90% of reference standard in enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.