Description

5-Maleimidovalericacid-Nhs is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a thiol-reactive maleimide group. This compound is essential for creating stable, site-specific conjugates between proteins, peptides, antibodies, and other biomolecules, enabling advanced research and diagnostic applications. It is a critical tool for scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and the creation of biosensors and immunoassays.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for attaching cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein/Peptide Labeling: For site-specific fluorescent or biotin labeling of thiol-containing biomolecules for imaging and detection.
• Surface Immobilization: Functionalizing gold surfaces, chips, or nanoparticles with proteins for biosensor and diagnostic device development.
• Polymer Modification: Introducing reactive maleimide groups into polymers or hydrogels for subsequent bioconjugation.
• Enzyme Immobilization: Covalently attaching enzymes to solid supports while aiming to preserve activity.
• Crosslinking Studies: Investigating protein-protein interactions or stabilizing protein complexes.

Basic Information

Product Name	5-Maleimidovalericacid-Nhs
CAS No.	103750-03-4
Molecular Formula	C14H16N2O7
Molecular Weight	324.29 g/mol
Synonyms	5-Maleimidovaleric Acid N-Hydroxysuccinimide Ester; 5-Maleimidopentanoic Acid N-Hydroxysuccinimide Ester; Maleimidocaproic Acid NHS Ester; 6-Maleimidocaproic Acid NHS Ester; ε-Maleimidocaproic Acid N-Succinimidyl Ester; SM(PEG)5 (as a common trade name analog); NHS-PEG5-Maleimide; 4-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)cyclohexane-1-carboxylic acid 2,5-dioxopyrrolidin-1-yl ester (IUPAC-like)
EINECS	Contact for details

Quality Control

Our 5-Maleimidovalericacid-Nhs is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive bioconjugation applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our clients in pharmaceutical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and the maleimide group can degrade upon exposure to moisture or nucleophiles. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.