Description

2-Chloro-4-Nitrophenyl-n-Acetylglucosaminide is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the precise detection and quantification of specific hydrolase activities, particularly N-acetyl-β-D-glucosaminidase (NAG). It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and clinical diagnostics sectors who require reliable and sensitive biochemical tools.

Application

• Enzymatic Substrate for NAG Assays: Primary chromogenic substrate for detecting and measuring N-acetyl-β-D-glucosaminidase (NAG) activity in research and diagnostic applications.
• Clinical Diagnostics: Used in the development of diagnostic kits for monitoring kidney function and detecting renal tubular damage.
• Biochemical Research: Essential tool for studying enzyme kinetics, inhibition, and the role of glycosidases in various biological pathways and disease models.
• Pharmaceutical Quality Control: Employed in purity and activity testing of enzyme preparations and related biopharmaceutical products.
• Microbiology Studies: Applied in assays to study bacterial or fungal glycosidase enzymes for identification and research purposes.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-n-Acetylglucosaminide
CAS No.	103614-82-0
Molecular Formula	C14H17ClN2O8
Molecular Weight	376.75 g/mol
Synonyms	2-Chloro-4-nitrophenyl N-acetyl-β-D-glucosaminide; 2-Chloro-4-nitrophenyl N-acetyl-β-D-glucosaminide; NAG-2CNP; 2CNP-NAG; 2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside; β-D-Glucopyranoside, 2-chloro-4-nitrophenyl 2-(acetylamino)-2-deoxy-; 4-Nitro-2-chlorophenyl N-acetyl-β-D-glucosaminide
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-n-Acetylglucosaminide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in suitable solvent

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.