Description

3-Indoxyl Phosphate, Di-p-Toluidinium Salt is a specialized chromogenic substrate salt widely used in enzymatic detection systems. Its primary value lies in its role as a key reagent for the colorimetric detection of alkaline phosphatase (ALP) activity, producing an insoluble blue indigo dye upon enzymatic cleavage. This compound is essential for researchers and manufacturers in the diagnostics, life sciences, and biotechnology sectors, particularly for applications in immunoassays, histochemistry, and molecular biology.

Application

• Diagnostic Assays: As a critical substrate in ELISA (Enzyme-Linked Immunosorbent Assay) and Western blotting for detecting alkaline phosphatase-labeled antibodies.
• Histology & Cytochemistry: Used in tissue staining (e.g., in situ hybridization, immunohistochemistry) to localize enzyme activity with high sensitivity.
• Molecular Biology Research: Employed in nucleic acid detection systems, including blotting techniques for DNA and RNA analysis.
• Microbiology: Applied in microbial detection kits that utilize alkaline phosphatase as a reporter enzyme.
• Biochemical Reagent: Serves as a standard reagent in enzymatic studies and for the development of new bioanalytical methods.
• Point-of-Care Testing (POCT): A component in the formulation of rapid diagnostic tests and lateral flow assays.

Basic Information

Product Name	3-Indoxyl Phosphate, Di-p-Toluidinium Salt
CAS No.	103404-81-5
Molecular Formula	C28H32N3O4P
Molecular Weight	505.55 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; BCIP p-Toluidine Salt; 5-Bromo-4-chloro-3-indoxyl phosphate, ditoluidine salt; Indoxyl phosphate, BCIP; BCIP; 3-Indolyl phosphate, 5-bromo-4-chloro-, bis(4-methylaniline) salt; Alkaline Phosphatase Substrate (BCIP); Chromogenic substrate for alkaline phosphatase.
EINECS	Contact for details

Quality Control

Our 3-Indoxyl Phosphate, Di-p-Toluidinium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant GMP and ISO 9001 guidelines for the production of fine chemicals for diagnostic and research use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may affect performance. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines
Performance Test	Produces characteristic blue color with alkaline phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.