Description

Hippuryl-His-Leu Acetate Salt is a synthetic tripeptide derivative widely utilized as a specific chromogenic substrate for the assay of angiotensin-converting enzyme (ACE) activity. This compound matters significantly in biochemical research and pharmaceutical development for its critical role in studying hypertension, cardiovascular diseases, and screening for ACE inhibitors. It is primarily needed by research institutions, pharmaceutical R&D laboratories, and diagnostic kit manufacturers focused on enzymatic studies and drug discovery processes.

Application

• ACE Activity Assays: Primary chromogenic substrate for the spectrophotometric determination of angiotensin-converting enzyme activity in biological samples.
• Drug Discovery & Screening: Essential tool for high-throughput screening (HTS) of potential ACE inhibitor drugs for treating hypertension and heart failure.
• Biochemical Research: Used in kinetic studies and mechanistic investigations of ACE enzyme function and regulation.
• Clinical Diagnostics: Component in diagnostic kits for measuring serum ACE levels, aiding in the diagnosis and monitoring of conditions like sarcoidosis and Gaucher's disease.
• Enzyme Characterization: Employed to study enzyme specificity, inhibition constants (Ki), and catalytic efficiency of ACE and related proteases.
• Quality Control in Pharma: Used as a reference standard in the quality control of biopharmaceutical products targeting the renin-angiotensin system.

Basic Information

Product Name	Hippuryl-His-Leu Acetate Salt
CAS No.	103404-54-2
Molecular Formula	C24H32N6O6 • xC2H4O2
Molecular Weight	500.55 g/mol (Free base)
Synonyms	Hippuryl-Histidyl-Leucine Acetate; N-Benzoylglycyl-L-histidyl-L-leucine Acetate Salt; ACE Substrate; Bz-Gly-His-Leu Acetate; HHL Acetate; Angiotensin Converting Enzyme Substrate; L-Hippuryl-L-histidyl-L-leucine acetate; Bz-Gly-His-Leu • AcOH
EINECS	Contact for details

Quality Control

Our Hippuryl-His-Leu Acetate Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for biological activity as an ACE substrate. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to ensure compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Assay (Enzymatic)	≥ 95% of theoretical activity
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.