Description

Gly-Pro p-Nitroanilide Hydrochloride is a specialized chromogenic peptide substrate used as a key reagent in enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of dipeptidyl peptidase (DPP) activity, particularly DPP-IV. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for drug discovery, biochemical research, and enzyme characterization studies.

Application

• Enzymatic Assay Substrate: Primary substrate for the colorimetric and spectrophotometric determination of dipeptidyl peptidase IV (DPP-IV/CD26) activity.
• Pharmaceutical Research: Used in high-throughput screening (HTS) assays to identify and characterize potential DPP-IV inhibitors for the treatment of type 2 diabetes and other metabolic disorders.
• Biochemical Research: Essential tool for studying the kinetics, specificity, and function of proline-specific peptidases in various biological pathways.
• Diagnostic Reagent Manufacturing: A key raw material in the production of diagnostic kits for clinical and research laboratories measuring DPP-IV activity.
• Quality Control (QC): Employed as a reference standard in QC testing for biopharmaceutical processes where DPP activity is monitored.

Basic Information

Product Name	Gly-Pro p-Nitroanilide Hydrochloride
CAS No.	103213-34-9
Molecular Formula	C13H17N5O4 • HCl
Molecular Weight	343.77 g/mol
Synonyms	Glycyl-L-proline p-nitroanilide hydrochloride; Gly-Pro-pNA • HCl; H-Gly-Pro-pNA • HCl; L-Glycyl-L-proline 4-nitroanilide hydrochloride; DPP-IV Substrate; Dipeptidyl Peptidase IV Substrate; GPpNA • HCl; 4-Nitroanilide of Glycylproline hydrochloride
EINECS	Contact for details

Quality Control

Our Gly-Pro p-Nitroanilide Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR, and verification of substrate activity. A detailed Certificate of Analysis (COA) documenting purity, assay, and physical properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Assay (Enzymatic)	Suitable for DPP-IV assays
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.