Description

4-Nitrophenyl 4,6-Cyclohexylidene-B-D-Mannopyranoside is a specialized synthetic glycoside derivative, serving as a critical chromogenic substrate for enzyme activity assays. Its core value lies in its high specificity and sensitivity for detecting and quantifying the activity of glycosidases, particularly α-mannosidases, in biochemical research. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries who require precise enzymatic analysis for drug discovery, biomarker research, and biochemical pathway studies.

Application

• Enzymatic Substrate for α-Mannosidase: Primary use as a chromogenic substrate for the spectrophotometric determination of α-mannosidase activity in vitro.
• Biochemical Research & Drug Discovery: Used in high-throughput screening (HTS) assays to identify and characterize enzyme inhibitors or activators for therapeutic development.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for measuring specific glycosidase levels, which can be indicators of certain genetic disorders (e.g., lysosomal storage diseases).
• Quality Control in Bioprocessing: Employed to monitor and ensure the consistency and activity of enzyme preparations used in industrial biocatalysis or biopharmaceutical production.
• Academic Research: Fundamental tool for studying glycosidase enzyme kinetics, mechanism of action, and substrate specificity in university and institutional labs.

Basic Information

Product Name	4-Nitrophenyl 4,6-Cyclohexylidene-B-D-Mannopyranoside
CAS No.	102717-16-8
Molecular Formula	C18H23NO8
Molecular Weight	381.38 g/mol
Synonyms	p-Nitrophenyl 4,6-O-cyclohexylidene-β-D-mannopyranoside; 4-Nitrophenyl 4,6-O-cyclohexylidene-β-D-mannoside; 4-NP-β-D-mannopyranoside 4,6-cyclohexylidene acetal; β-D-Mannopyranoside, 4-nitrophenyl 4,6-O-cyclohexylidene-; Chromogenic α-mannosidase substrate; pNP-β-D-Mannopyranoside cyclohexylidene derivative
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 4,6-Cyclohexylidene-B-D-Mannopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical assays. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and reliability for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after each use to prevent degradation from atmospheric moisture. For long-term storage, consider using desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.