Description

n-Cbz-Gly-Pro-Arg p-Nitro-Anilide Acetate Salt is a specialized chromogenic peptide substrate designed for the sensitive detection and quantification of serine protease activity, particularly thrombin. Its core value lies in providing a reliable, colorimetric readout for enzymatic assays, enabling critical research and quality control in life sciences. This compound is essential for researchers and manufacturers in pharmaceutical development, clinical diagnostics, and biochemical research who require precise tools for studying blood coagulation pathways and protease kinetics.

Application

• Thrombin Activity Assays: Primary substrate for the colorimetric determination of thrombin concentration and activity in research and diagnostic kits.
• Serine Protease Research: Used in kinetic studies and inhibitor screening for enzymes like trypsin, plasmin, and other trypsin-like proteases.
• Pharmaceutical QC: Employed in the quality control of heparin and other anticoagulant drugs by measuring residual thrombin activity.
• Blood Coagulation Studies: A key tool for investigating the coagulation cascade, factor Xa activity, and related hemostatic disorders.
• Diagnostic Reagent Manufacturing: Integrated as a critical component in the production of in-vitro diagnostic (IVD) reagents for thrombophilia testing.
• Enzyme Characterization: Facilitates the determination of kinetic parameters (Km, Vmax) for protease characterization.

Basic Information

Product Name	n-Cbz-Gly-Pro-Arg p-Nitro-Anilide Acetate Salt
CAS No.	102679-70-9
Molecular Formula	C30H39N9O9 • xC2H4O2
Molecular Weight	669.70 g/mol (Free base)
Synonyms	Benzyloxycarbonyl-Glycyl-Prolyl-Arginine 4-Nitroanilide Acetate Salt; Z-Gly-Pro-Arg-pNA • AcOH; Chromogenic Substrate S-2238; H-D-Phe-Pip-Arg-pNA • 2HCl; Thrombin Substrate I; Bz-Phe-Val-Arg pNA • HCl; CBZ-Gly-Pro-Arg-pNA Acetate; Tosyl-Gly-Pro-Arg p-Nitroanilide Acetate
EINECS	Contact for details

Quality Control

Our n-Cbz-Gly-Pro-Arg p-Nitro-Anilide Acetate Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive enzymatic assays. Each lot is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and spectral data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at low temperature. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Assay (UV-Vis)	95.0 - 105.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.