Description

5-Cyano-2,3-Bis(3-Cyanophenyl)-2H-Tetrazolium Chloride is a specialized tetrazolium salt primarily used as a vital reagent in biochemical and analytical chemistry. Its core value lies in its role as a precursor for formazan dyes, which are critical for colorimetric assays that measure cellular metabolic activity and enzyme function. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries developing cell viability tests, such as MTT and related assays.

Application

• Cell Viability & Proliferation Assays: Key precursor for generating formazan dyes in colorimetric assays like MTT, used extensively in drug discovery and toxicology studies.
• Biochemical Research: Employed as an electron acceptor in dehydrogenase enzyme activity studies and other redox-based biochemical tests.
• Diagnostic Kit Manufacturing: Used in the formulation of commercial diagnostic kits for clinical and research laboratories to assess cell health and metabolic function.
• Pharmaceutical Development: Applied in high-throughput screening (HTS) platforms to evaluate the cytotoxic or cytostatic effects of new drug candidates.
• Microbiology: Utilized in assays to study microbial cell viability and metabolic activity.
• Material Science Research: Investigated for potential applications in developing novel electrochemical sensors and imaging agents.

Basic Information

Product Name	5-Cyano-2,3-Bis(3-Cyanophenyl)-2H-Tetrazolium Chloride
CAS No.	102568-49-0
Molecular Formula	C22H12ClN9
Molecular Weight	437.85 g/mol
Synonyms	2H-Tetrazolium, 5-cyano-2,3-bis(3-cyanophenyl)-, chloride; 5-Cyano-2,3-di-m-cyanophenyltetrazolium Chloride; CTC; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide Analog; Tetrazolium Chloride Derivative; MTT Tetrazolium Salt Analog; Cell Viability Assay Reagent; Formazan Dye Precursor
EINECS	Contact for details

Quality Control

Every batch of 5-Cyano-2,3-Bis(3-Cyanophenyl)-2H-Tetrazolium Chloride is manufactured and tested under a strict quality management system. We provide comprehensive analytical documentation, including a Certificate of Analysis (COA) detailing purity, identity, and impurity profiles to ensure it meets the exacting standards required for sensitive biochemical applications. Our quality protocols are designed to support GMP/GLP-compliant research and production.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment and handled under low-light conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.