Description

Bis(Sulfo-n-Succinimidyl)Doxyl-2-Spiro-4'-Pimelate is a specialized, water-soluble, heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysulfosuccinimide (NHS) ester and a nitroxide spin label (doxyl). This compound is essential for creating stable covalent linkages between biomolecules while enabling advanced structural and dynamic studies via electron paramagnetic resonance (EPR) spectroscopy. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, structural biology, and pharmaceutical development who require precise, site-specific labeling of proteins, peptides, and other amine-containing compounds.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Covalently attaches a stable nitroxide radical to proteins to study protein folding, dynamics, and conformational changes.
• Bioconjugation and Protein-Protein Crosslinking: Creates stable amide bonds between primary amines, enabling the conjugation of antibodies, enzymes, or other biomolecules for diagnostic and therapeutic applications.
• Membrane Protein Studies: Used to label and probe the structure and environment of membrane-embedded proteins.
• Polymer and Material Science Functionalization: Introduces spin labels onto amine-functionalized polymers or nanoparticles for material characterization and tracking.
• Development of Molecular Rulers: The defined spacer length of the pimelate moiety makes it useful for distance measurements in structural biology.
• Immobilization of Biomolecules: Facilitates the covalent attachment of proteins or peptides to amine-reactive solid supports for biosensor or affinity chromatography development.

Basic Information

Product Name	Bis(Sulfo-n-Succinimidyl)Doxyl-2-Spiro-4'-Pimelate
CAS No.	102260-45-7
Molecular Formula	C27H34N4O14S2 · xNa
Molecular Weight	694.70 g/mol (Free acid)
Synonyms	BS3-Doxyl; BS3-Doxyl; Bis(sulfosuccinimidyl) doxyl-2-spiro-4'-pimelate; Sodium Bis(sulfosuccinimidyl) doxyl-2-spiro-4'-pimelate; Doxyl BS3; SULFO-DOXYL-BS3; 3,3'-(7-(1-Oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)heptane-1,7-diyl)bis(1-(3-sulfonatopropyl)-2,5-dioxopyrrolidine-3-sulfonate); Heterobifunctional NHS-ester spin label.
EINECS	Contact for details

Quality Control

Our Bis(Sulfo-n-Succinimidyl)Doxyl-2-Spiro-4'-Pimelate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in water, DMSO
Functional Activity	EPR active; Conjugation efficiency confirmed
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.