Description

n-Formyl-Methionine p-Nitroanilide is a specialized biochemical reagent primarily used as a chromogenic substrate for enzymatic assays. This compound is critical for research and quality control in life sciences, enabling precise measurement of protease and peptidase activity. It is essential for pharmaceutical R&D, diagnostic kit manufacturing, and academic research in biochemistry and molecular biology.

Application

• Protease/Peptidase Substrate: A key chromogenic substrate for the kinetic and endpoint assay of various proteolytic enzymes, including methionine aminopeptidases.
• Pharmaceutical Research: Used in high-throughput screening (HTS) to identify and characterize enzyme inhibitors for drug discovery programs.
• Diagnostic Manufacturing: Employed in the formulation of diagnostic kits for clinical analysis of specific enzyme levels.
• Biochemical Research: Facilitates studies on enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial laboratories.
• Quality Control: Serves as a reference standard in the quality assurance of enzymatic reagents and diagnostic products.

Basic Information

Product Name	n-Formyl-Methionine p-Nitroanilide
CAS No.	102185-40-0
Molecular Formula	C12H15N3O4S
Molecular Weight	297.33 g/mol
Synonyms	N-Formyl-L-methionine 4-nitroanilide; Formyl-Met-pNA; F-Met-pNA; N-Formylmethionine p-nitroanilide; L-Methionine, N-formyl-, 4-nitroanilide; Methionine, N-formyl-, 4-nitroanilide, L-; Substrate for methionine aminopeptidase; 4-Nitroanilide of N-formyl-L-methionine
EINECS	Contact for details

Quality Control

Our n-Formyl-Methionine p-Nitroanilide is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and verification of physical properties. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive (store away from light). For long-term storage, desiccate at 2-8°C.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.