Description

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt is a chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its enzymatic conversion to an insoluble, intensely colored indigo dye, enabling precise detection and quantification. This compound is essential for researchers and manufacturers in the life sciences, particularly for applications in blotting techniques, immunoassays, and histochemistry.

Application

• Western Blotting (Immunoblotting): As a substrate for alkaline phosphatase (AP)-conjugated antibodies to visualize specific proteins.
• Enzyme-Linked Immunosorbent Assay (ELISA): For colorimetric detection in AP-based ELISA kits and diagnostic test systems.
• Nucleic Acid Hybridization: Used in Southern and Northern blotting for the detection of DNA or RNA with AP-labeled probes.
• In Situ Hybridization (ISH): For the localization of specific nucleic acid sequences in tissue sections or cell preparations.
• Lateral Flow Assays: As a key component in rapid diagnostic tests requiring a visual color readout.
• Histochemical Staining: To localize alkaline phosphatase activity in tissue samples for research and pathology.
• Phosphatase Activity Assays: For the biochemical measurement of alkaline phosphatase enzyme activity in various samples.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt
CAS No.	102185-33-1
Molecular Formula	C8H5BrClNNa2O4P
Molecular Weight	369.44 g/mol
Synonyms	BCIP disodium salt; 5-Bromo-4-chloro-3-indolyl phosphate disodium salt; X-Phosphate disodium salt; BCIP, Na2 salt; 5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate disodium salt; 3-Indolyl phosphate, 5-bromo-4-chloro-, disodium salt; BCIP substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee compliance with your research and manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Solubility	Soluble in water
Color Reaction	Yields intense blue-purple precipitate with Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.