Description

Ala-Ala-Val-Ala p-Nitro-Anilide is a specialized synthetic peptide substrate designed for enzymatic activity studies. This compound is critical for researchers and manufacturers requiring precise, high-purity biochemical tools for protease and peptidase research. It is primarily utilized in pharmaceutical R&D, diagnostic assay development, and biochemical research laboratories focused on enzyme kinetics and inhibitor screening.

Application

• Protease/Peptidase Substrate: A specific chromogenic substrate for the kinetic analysis and characterization of various serine and cysteine proteases.
• Pharmaceutical Research: Used in high-throughput screening (HTS) assays to identify and evaluate potential enzyme inhibitors for drug discovery programs.
• Diagnostic Development: Employed in the development of colorimetric or fluorometric diagnostic kits for detecting specific enzymatic activities.
• Biochemical Mechanism Studies: Essential for studying enzyme specificity, catalytic mechanisms, and structure-activity relationships (SAR) in academic and industrial settings.
• Quality Control in Biologics: Can be used to assess the activity and consistency of enzyme-based therapeutic products during manufacturing.

Basic Information

Product Name	Ala-Ala-Val-Ala p-Nitro-Anilide
CAS No.	102185-27-3
Molecular Formula	C22H33N7O7
Molecular Weight	507.54 g/mol
Synonyms	AAVA pNA; L-Alanyl-L-alanyl-L-valyl-L-alanine 4-nitroanilide; AAVA-pNA; Ala-Ala-Val-Ala-pNA; Tetrapeptide substrate AAVA-pNA; N-Succinyl-Ala-Ala-Val-Ala-p-nitroanilide (common related form); Protease Substrate IV (contextual); Suc-AAVA-pNA (common related form)
EINECS	Contact for details

Quality Control

Our Ala-Ala-Val-Ala p-Nitro-Anilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification (NMR, MS), to ensure it meets the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.