Description

Azocasein is a chemically modified derivative of the milk protein casein, where diazonium groups are introduced, making it a valuable chromogenic substrate for proteolytic enzyme assays. This compound is essential for researchers and quality control laboratories requiring precise, colorimetric measurement of protease activity. It is primarily utilized in the biotechnology, pharmaceutical, and food science industries for enzyme kinetics studies, screening for protease inhibitors, and quality control of enzymatic products.

Application

• Protease Activity Assays: A standard chromogenic substrate for the quantitative determination of trypsin, chymotrypsin, papain, and other serine and cysteine proteases.
• Enzyme Kinetics & Inhibition Studies: Used in biochemical research to study enzyme mechanisms, calculate kinetic parameters (Km, Vmax), and screen for potential protease inhibitors.
• Pharmaceutical Quality Control: Employed in the testing and standardization of therapeutic enzymes and in-process monitoring during biopharmaceutical production.
• Food & Beverage Industry: Applied to analyze protease activity in brewing, baking, meat tenderization, and dairy processing.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for measuring proteolytic activity in clinical samples.
• Microbiological & Cell Culture Research: Used to assess protease secretion by microorganisms or cultured cells.

Basic Information

Product Name	Azocasein
CAS No.	102110-74-7
Molecular Formula	N/A (Protein derivative)
Molecular Weight	N/A (Variable)
Synonyms	Azocasein; Azo-casein; Diazo Casein; Casein, azobenzeneazocarbonyl derivative; Succinylated casein, azobenzeneazocarbonyl derivative; Protease substrate azocasein; Chromogenic casein
EINECS	Contact for details

Quality Control

Our Azocasein is produced and tested to ensure high lot-to-lot consistency and performance in enzymatic assays. Each batch undergoes rigorous quality testing, including assessment of solubility, chromogenic response, and suitability for specific protease assays. A comprehensive Certificate of Analysis (COA) detailing purity, performance data, and microbiological limits is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Orange to brownish powder
Identification (IR)	Conforms to structure
Solubility (1% in buffer)	Complete, clear to slightly opalescent solution
Moisture Content (Loss on Drying)	≤ 8.0 %
Assay (Protease Substrate Activity)	Conforms to reference standard
Microbiological Total Plate Count	≤ 1000 CFU/g
Yeast & Mold	≤ 100 CFU/g

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.