Description

Resorufin β-D-Glucopyranoside is a specialized fluorogenic substrate used for the detection and quantification of β-glucosidase enzyme activity. This compound is critical for researchers and quality control laboratories requiring high-sensitivity enzymatic assays. It is primarily utilized in the biotechnology, pharmaceutical, and diagnostic industries for applications ranging from enzyme kinetics studies to high-throughput screening.

Application

• Enzymatic Assay Substrate: A key fluorogenic substrate for the sensitive detection and kinetic analysis of β-glucosidase enzymes.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for genetic disorders like Gaucher's disease, where β-glucosidase activity is a key biomarker.
• Pharmaceutical Research & HTS: Employed in high-throughput screening (HTS) campaigns to identify modulators (inhibitors or activators) of β-glucosidase for drug discovery.
• Microbiology & Cell Biology: Applied as a reporter substrate in studies of microbial enzyme production, gene expression, and cellular processes involving glycoside hydrolases.
• Biomass Conversion Research: Utilized in biofuel research to assay cellulase and other lignocellulose-degrading enzyme complexes that exhibit β-glucosidase activity.
• Food & Beverage Quality Control: Acts as a substrate in enzymatic assays to monitor β-glucosidase activity relevant to flavor development in products like wine and fruit juices.

Basic Information

Product Name	Resorufin β-D-Glucopyranoside
CAS No.	101490-85-1
Molecular Formula	C19H17NO8
Molecular Weight	387.34 g/mol
Synonyms	Resorufin β-D-Glucopyranoside; Resorufin β-D-Glucoside; Resorufin β-Glucoside; 7-Hydroxy-3H-phenoxazin-3-one 1-β-D-glucopyranoside; β-Glucosidase Substrate Resorufin; 1-β-D-Glucopyranosyloxy-7-hydroxyphenoxazin-3-one; Resorufin Glucuronide (common misnomer, structurally related); RG (abbreviation)
EINECS	Contact for details

Quality Control

Our Resorufin β-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage under inert atmosphere, please contact our technical team for specific recommendations.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Conforms to functional assay standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.