Description

4-Nitrophenyl-(Alpha)-Mannopyranoside 6-(α-Glucopyranosyl Phosphate) is a specialized synthetic glycoside derivative designed for advanced biochemical research and diagnostic applications. This compound serves as a critical chromogenic substrate for the sensitive detection and kinetic study of specific glycosyltransferase and glycosidase enzymes. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers requiring high-purity, well-characterized enzyme substrates to ensure accurate and reproducible experimental results.

Application

• Enzyme Substrate for Glycosyltransferases: Used as a specific acceptor substrate in assays to study the activity of enzymes like mannosyltransferases and glucosyltransferases involved in glycoconjugate biosynthesis.
• Glycosidase Activity Profiling: Acts as a chromogenic substrate for α-mannosidases and related hydrolytic enzymes, releasing 4-nitrophenol for convenient spectrophotometric detection.
• Biochemical Pathway Research: Essential for in vitro studies of carbohydrate metabolism, oligosaccharide synthesis, and the biochemical characterization of carbohydrate-active enzymes (CAZymes).
• Diagnostic Kit Development: Incorporated into enzymatic assay kits for clinical diagnostics and research to measure specific enzyme activities linked to metabolic disorders or cellular processes.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery programs to screen for inhibitors or activators of target glycosyltransferases as potential therapeutic agents.
• Glycobiology Research: A key tool for investigating enzyme specificity, kinetics, and inhibition mechanisms within the field of glycobiology.

Basic Information

Product Name	4-Nitrophenyl-(Alpha)-Mannopyranoside 6-(α-Glucopyranosyl Phosphate)
CAS No.	101455-34-9
Molecular Formula	C18H26NO16P
Molecular Weight	519.37 g/mol
Synonyms	p-Nitrophenyl α-D-mannopyranoside 6-(α-D-glucopyranosyl phosphate); 4-Nitrophenyl α-D-mannopyranoside 6-(α-D-glucopyranosyl phosphate); α-D-Glucopyranosyl α-D-mannopyranosyl-(1→6)-phosphate 4-nitrophenyl ester; PNP-α-Man-6-Glc-α-P; Chromogenic mannosyltransferase substrate; 4-Nitrophenol mannoside glucosyl phosphate
EINECS	Contact for details

Quality Control

Every batch of 4-Nitrophenyl-(Alpha)-Mannopyranoside 6-(α-Glucopyranosyl Phosphate) is produced and analyzed under strict quality management protocols to ensure identity, purity, and performance consistency. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity (typically by HPLC), identity confirmation (NMR, MS), and specific performance in enzymatic assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Enzymatic Activity	Validated with reference enzyme

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.