Description

H-Phe-Arg-βna · 2 Hcl is a synthetic peptide derivative, specifically a dipeptide amide with a benzylamine (Bna) capping group, supplied as a dihydrochloride salt. This compound is a crucial building block and research chemical in advanced peptide synthesis and biochemical studies. It is primarily utilized by pharmaceutical R&D laboratories, biotechnology companies, and academic institutions focused on protease substrate development, enzyme kinetics, and medicinal chemistry.

Application

• Protease Substrate Research: Used as a selective and sensitive fluorogenic or chromogenic substrate for studying the activity and inhibition of specific proteases, such as trypsin-like enzymes.
• Biochemical Assay Development: Serves as a key component in developing high-throughput screening (HTS) assays for drug discovery targeting proteolytic pathways.
• Medicinal Chemistry & Peptide Design: Acts as an advanced intermediate for constructing more complex peptide-based inhibitors, probes, or therapeutic candidates.
• Enzyme Mechanism Studies: Employed in kinetic analyses to elucidate cleavage specificity and catalytic efficiency of serine or cysteine proteases.
• Diagnostic Reagent Formulation: Can be incorporated into diagnostic kits for detecting and quantifying specific protease activities in clinical or research samples.

Basic Information

Item	Details
Product Name	H-Phe-Arg-βna · 2 Hcl
CAS No.	100929-99-5
Molecular Formula	C26H38Cl2N8O3
Molecular Weight	581.54 g/mol
Synonyms	Phe-Arg-βna dihydrochloride; H-Phe-Arg-4-nitroanilide dihydrochloride (common); L-Phenylalanyl-L-arginyl-4-nitroanilide dihydrochloride; Boc-Phe-Arg-βna · 2 HCl (deprotected form); F-R-βna · 2 HCl; Protease Substrate IV (related); Benzoyl-Phe-Arg-4-nitroanilide derivative; Synthetic peptide substrate 100929-99-5
EINECS	Contact for details

Quality Control

Every batch of H-Phe-Arg-βna · 2 Hcl is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality assurance protocol aligns with research-grade and potential cGMP standards for non-therapeutic applications. A comprehensive Certificate of Analysis (COA) detailing purity (HPLC), identity (MS, NMR), and impurity profiles is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage over 12 months, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	< 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines
Assay (Titration)	97.0% - 103.0% (on anhydrous basis)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.