Description

4-Heptylumbelliferyl-β-Glucoside is a synthetic fluorogenic substrate specifically designed for the detection and quantification of β-glucosidase enzyme activity. This compound matters as a critical diagnostic and research reagent, offering high sensitivity and specificity for enzymatic assays. It is primarily needed by professionals in life science research, pharmaceutical development, and industrial biotechnology for applications in enzyme kinetics, high-throughput screening, and diagnostic kit formulation.

Application

• Enzymatic Assay Substrate: A key fluorogenic reagent for the sensitive detection and kinetic analysis of β-glucosidase enzymes in research and diagnostic laboratories.
• High-Throughput Screening (HTS): Used in pharmaceutical and biotechnology discovery programs to identify inhibitors or activators of β-glucosidase for drug development.
• Diagnostic Kit Component: Incorporated into clinical and biochemical test kits for the diagnosis of genetic disorders like Gaucher's disease, where β-glucosidase activity is measured.
• Microbiological Research: Employed to study β-glucosidase-producing microorganisms in environmental and industrial microbiology.
• Plant Biochemistry Studies: Utilized to investigate the role of β-glucosidases in plant defense mechanisms and metabolic pathways.
• Quality Control in Enzyme Production: Serves as a standard substrate for assessing the purity and activity of commercially produced β-glucosidase enzymes.

Basic Information

Product Name	4-Heptylumbelliferyl-β-Glucoside
CAS No.	100443-43-4
Molecular Formula	C22H30O9
Molecular Weight	438.47 g/mol
Synonyms	4-Heptylumbelliferyl β-D-Glucopyranoside; 4-Heptylumbelliferyl β-D-Glucoside; 4-Heptyl-7-hydroxycoumarin β-D-Glucopyranoside; HUBG; 4-Heptyl-7-β-D-glucopyranosyloxycoumarin; β-Glucosidase Substrate (Heptylumbelliferyl); 4-MU-β-Glc(C7) (informal); Contact for additional trade names.
EINECS	Contact for details

Quality Control

Our 4-Heptylumbelliferyl-β-Glucoside is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant industry guidelines for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.