Description

2-Nitrophenylgalactoside is a specialized chromogenic substrate used in enzymatic assays and biochemical research. Its core value lies in its ability to act as a specific substrate for galactosidase enzymes, producing a measurable colorimetric change upon cleavage. This makes it an essential reagent for researchers and quality control laboratories in the life sciences, pharmaceutical development, and diagnostic manufacturing sectors.

Application

• Enzymatic Assay Substrate: Primary use as a chromogenic substrate for β-galactosidase in molecular biology and biochemistry.
• Diagnostic Reagent Manufacturing: A key component in the production of test kits for enzyme-linked immunosorbent assays (ELISA) and other clinical diagnostics.
• Microbiology Research: Used in microbial identification and gene expression studies, particularly in reporter gene assays (e.g., lacZ gene).
• Pharmaceutical R&D: Employed in high-throughput screening (HTS) to identify and characterize enzyme inhibitors or activators.
• Food & Beverage Analysis: Potential application in enzymatic methods for detecting specific sugars or microbial activity.
• Biochemical Standard: Serves as a reference standard for calibrating enzymatic activity measurements.

Basic Information

Product Name	2-Nitrophenylgalactoside
CAS No.	19710-96-4
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	o-Nitrophenyl-β-D-galactopyranoside; ONPG; 2-Nitrophenyl β-D-galactopyranoside; β-D-Galactopyranoside, 2-nitrophenyl; o-Nitrophenylgalactoside; ONP-Gal; 4-Nitrophenyl-β-D-galactopyranoside (common related compound); β-Galactosidase substrate
EINECS	243-259-6

Quality Control

Our 2-Nitrophenylgalactoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light); therefore, containers must be kept sealed in a desiccated environment to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO
Absorbance (Lambda max)	~420 nm (upon enzymatic cleavage)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.