Description

Z-Phe-Pna is a protected dipeptide building block, specifically N-benzyloxycarbonyl-L-phenylalanyl p-nitroanilide. This compound is a critical reagent in peptide synthesis and biochemical research, serving as a chromogenic substrate for the assay of proteolytic enzymes such as chymotrypsin and subtilisin. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors who require high-purity, reliable substrates for enzyme kinetics studies, inhibitor screening, and diagnostic assay development.

Application

• Protease/Chymotrypsin Substrate: Primary use as a sensitive chromogenic substrate for the spectrophotometric determination of chymotrypsin, subtilisin, and other serine proteases.
• Enzyme Kinetics & Inhibition Studies: Used in biochemical research to study enzyme mechanisms, measure kinetic parameters (Km, Vmax), and screen for potential protease inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits and reagents for clinical and research laboratories.
• Peptide Synthesis Intermediate: Serves as a protected amino acid derivative in solid-phase and solution-phase peptide synthesis.
• Quality Control in Bioprocessing: Employed to monitor and validate the activity of protease enzymes used in industrial bioprocesses.

Basic Information

Product Name	Z-Phe-Pna
CAS No.	19647-71-3
Molecular Formula	C23H21N3O5
Molecular Weight	419.43 g/mol
Synonyms	N-Cbz-L-Phenylalanine p-Nitroanilide; Z-L-Phenylalanine p-nitroanilide; N-Benzyloxycarbonyl-L-phenylalanyl 4-nitroanilide; Nα-Carbobenzoxy-L-phenylalanine 4-nitroanilide; Chymotrypsin Substrate; Suc-Ala-Ala-Pro-Phe pNA; Z-Phe-pNA; Z-Phe-4-NA; CBZ-Phe-pNA
EINECS	Contact for details

Quality Control

Our Z-Phe-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification (IR, NMR), and specific optical rotation verification to ensure high chemical and chiral purity suitable for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	-15.0° to -18.0° (c=1 in DMF)
Melting Point	198°C - 202°C
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.