Description

Bicine is a high-purity, zwitterionic biological buffer essential for maintaining stable pH conditions in sensitive biochemical and molecular biology applications. Its excellent buffering capacity in the pH range of 7.6-9.0 makes it a critical reagent for ensuring experimental reproducibility and system stability. This product is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic kit formulation sectors.

Application

• Biochemical Research & Diagnostics: As a primary buffer component in enzyme assays, cell culture media, and electrophoresis (e.g., SDS-PAGE).
• Pharmaceutical Formulation: Used in the development and stabilization of protein-based therapeutics, vaccines, and diagnostic reagents.
• Molecular Biology: Employed in nucleic acid purification, PCR, and other procedures requiring precise, metal-chelating buffer systems.
• Industrial Bioprocessing: Acts as a buffering agent in fermentation processes and downstream purification of biomolecules.

Basic Information

Product Name	Bicine
CAS No.	150-25-4
Molecular Formula	C6H13NO4
Molecular Weight	163.17 g/mol
Synonyms	N,N-Bis(2-hydroxyethyl)glycine; Dihydroxyethylglycine; BIS(2-HYDROXYETHYL)AMINOACETIC ACID; 2,2'-[(Carboxymethyl)imino]diethanol; Glycine, N,N-bis(2-hydroxyethyl)-; BICINE buffer; Good's buffer
EINECS	205-753-9

Quality Control

Our Bicine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including assay, identification, and impurity profiling. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); keep the container tightly sealed to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	7.5 - 8.5
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.