Description

3-Doxyl-5Alpha-Cholestane, Free Radical is a nitroxide spin-labeled derivative of cholesterol, widely recognized as a stable free radical probe. This compound is essential for studying membrane dynamics, lipid-protein interactions, and molecular mobility using Electron Paramagnetic Resonance (EPR) spectroscopy. It is a critical research tool for scientists and R&D departments in the pharmaceutical, biophysical research, and advanced materials sectors.

Application

• Membrane Biophysics Research: As a spin label incorporated into lipid bilayers and cell membranes to investigate fluidity, phase transitions, and domain formation.
• Protein-Lipid Interaction Studies: Used to probe the localization and dynamics of cholesterol around membrane proteins and receptors.
• EPR Spectroscopy Standard: Serves as a stable radical standard for instrument calibration and method development in spin-labeling techniques.
• Model System for Oxidative Stress: Employed in studies modeling free radical processes and antioxidant efficacy in lipid environments.
• Materials Science: Applied in the development of advanced materials where controlled radical processes or specific molecular ordering is required.
• Drug Delivery Research: Utilized to trace and understand the behavior of liposomal and other lipid-based drug delivery systems.

Basic Information

Product Name	3-Doxyl-5Alpha-Cholestane, Free Radical
CAS No.	18353-76-9
Molecular Formula	C₃₄H₅₈NO₂
Molecular Weight	512.84 g/mol
Synonyms	3-Doxyl-5α-cholestane; 5α-Cholestan-3β-yloxy-2,2-dimethyloxazolidin-4-yl; 3-(2,2-Dimethyl-1-oxido-4-oxazolidinyl)-5α-cholestane; 5α-Cholestan-3β-yloxy-2,2-dimethyl-4-oxazolidinyloxy; 3-Carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy-5α-cholestane (related structure); Cholestane Spin Label; Cholesterol Analog Spin Probe; Nitroxide-labeled Cholesterol; EPR Spin Label for Membranes
EINECS	Contact for details

Quality Control

Our 3-Doxyl-5Alpha-Cholestane is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, critical for sensitive spectroscopic applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and EPR spectroscopy to confirm identity, purity, and radical stability. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation of the nitroxide radical. Keep the container in a dry, cool, and well-ventilated place. For long-term stability, desiccate if necessary.

Specification

Item	Specification
Appearance	Off-white to pale yellow crystalline solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
EPR Signal Intensity	Conforms to standard
Melting Point	Contact for details
Loss on Drying	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.