Description

Ac-Phe-Pna is a protected amino acid derivative, specifically an N-acetylated phenylalanine para-nitroanilide. This compound serves as a critical synthetic intermediate and a specialized substrate for enzymatic studies, particularly in protease research. It is primarily utilized by research institutions and pharmaceutical companies engaged in peptide synthesis and biochemical assay development.

Application

• Protease/Enzyme Substrate: Used as a chromogenic or fluorogenic substrate for the assay and characterization of various proteolytic enzymes, including chymotrypsin and subtilisin.
• Peptide Synthesis Intermediate: Acts as a key building block in solid-phase and solution-phase peptide synthesis (SPPS) for constructing more complex peptide sequences.
• Biochemical Research: Employed in kinetic studies, inhibitor screening, and mechanistic investigations of enzyme activity and specificity.
• Pharmaceutical Development: Utilized in the research and development stages of novel therapeutic agents targeting protease pathways.
• Diagnostic Reagent Manufacturing: Serves as a component in the formulation of diagnostic kits and reagents for clinical and research laboratories.

Basic Information

Product Name	Ac-Phe-Pna
CAS No.	17682-83-6
Molecular Formula	C₁₇H₁₇N₃O₄
Molecular Weight	327.34 g/mol
Synonyms	N-Acetyl-L-phenylalanine p-nitroanilide; Acetylphenylalanyl p-nitroaniline; Ac-Phe-pNA; L-Phenylalanine, N-acetyl-, 4-nitroanilide; N-Acetyl-L-phenylalanine 4-nitroanilide; Protease Substrate; Chymotrypsin Substrate; Suc-Phe-pNA (Note: Often confused, but Suc- is succinyl, not acetyl); Acetyl-Phe-pNA
EINECS	Contact for details

Quality Control

Every batch of Ac-Phe-Pna CAS NO 17682-83-6 is produced under a strict quality management system. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for research and development applications. A detailed Certificate of Analysis (COA), including results for identity, purity, and related substances, is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.2%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.