Description

6-Thioguanosine 5'-Triphosphate is a modified nucleotide analog where the oxygen atom at the 6-position of guanine is replaced by sulfur. This compound is a critical biochemical reagent, primarily serving as a substrate for RNA polymerases and reverse transcriptases in enzymatic synthesis and research applications. It is essential for scientists and manufacturers in the fields of molecular biology, biochemistry, and pharmaceutical research, particularly for studying nucleic acid metabolism, enzyme mechanisms, and developing diagnostic assays.

Application

• Enzymatic RNA Synthesis: Acts as a substrate for T7 RNA polymerase and other polymerases for the in vitro synthesis of thio-modified RNA transcripts.
• Biochemical Research: Used in studies of nucleotide metabolism, enzyme kinetics, and the mechanisms of RNA polymerases and reverse transcriptases.
• SELEX (Systematic Evolution of Ligands by EXponential Enrichment): Incorporated into RNA libraries to generate thio-modified aptamers with enhanced nuclease resistance and binding properties.
• Diagnostic Assay Development: Employed in the creation of labeled probes and substrates for sensitive detection systems.
• Structural Studies: Useful for X-ray crystallography and NMR studies of protein-nucleic acid interactions due to the heavy atom effect of sulfur.
• Antiviral & Anticancer Research: Investigated for its potential to inhibit viral replication or cellular processes as a nucleotide analog.

Basic Information

Product Name	6-Thioguanosine 5'-Triphosphate
CAS No.	17670-19-8
Molecular Formula	C10H16N5O13P3S
Molecular Weight	539.22 g/mol
Synonyms	6-Thio-GTP; 6-Thioguanosine triphosphate; GTP-γ-S (analog); Guanosine-5'-triphosphate, 6-thio-; 6-Mercaptoguanosine 5'-triphosphate; s6GTP; 6-SH-GTP
EINECS	Contact for details

Quality Control

Our 6-Thioguanosine 5'-Triphosphate is produced and tested under stringent quality management systems. Each batch is analyzed to ensure high purity and consistency, with specifications typically meeting the requirements for molecular biology and biochemical research. Comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment to guarantee traceability and support your regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0 %
Solubility	Soluble in water or buffer solutions
Biological Activity	Suitable for enzymatic reactions (e.g., with T7 RNA polymerase)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.