Description

5-Bromo-4-Chloro-3-Indoxyl-β-D-Fucopyranoside is a specialized chromogenic substrate used in molecular biology and diagnostic applications. This compound is critical for the specific detection of enzymatic activity, particularly β-fucosidase, providing a clear visual or fluorescent signal upon cleavage. It is an essential reagent for researchers and manufacturers in the fields of biochemical research, diagnostic kit development, and microbiology.

Application

• Chromogenic Substrate for β-Fucosidase: Primary use in enzymatic assays for the detection and quantification of β-fucosidase activity.
• Microbiological Identification: Used in culture media and diagnostic plates to differentiate bacterial species based on specific enzyme profiles.
• Biochemical Research: A key tool for studying glycosidase enzymes, enzyme kinetics, and metabolic pathways in vitro.
• Diagnostic Kit Manufacturing: Incorporated into ready-to-use kits for clinical diagnostics and food safety testing.
• Histochemistry and Cell Staining: Employed in techniques to localize enzyme activity within tissues and cells.
• High-Throughput Screening (HTS): Suitable for automated screening platforms in pharmaceutical discovery for identifying enzyme inhibitors or activators.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl-β-D-Fucopyranoside
CAS No.	17016-46-5
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl β-D-fucopyranoside; X-Fuc; BCIF; 5-Br-4-Cl-3-indoxyl-β-D-fucoside; β-Fucosidase Substrate; 3-Indolyl-β-D-fucopyranoside, 5-bromo-4-chloro derivative; 17016-46-5
EINECS	Contact for details

Quality Control

Every batch of 5-Bromo-4-Chloro-3-Indoxyl-β-D-Fucopyranoside is manufactured and tested under strict quality management systems. We ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 20 ppm
Residue on Ignition	< 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.