Description

4-Phenylazobenzyloxycarbonyl-L-Pro-Leu-Gly-Pro-D-Arg Dihydrate is a specialized, chromophore-labeled peptide derivative primarily used as a key substrate in biochemical research and assay development. This compound matters for its role in studying enzyme kinetics and specificity, particularly for proteases like collagenase and other matrix metalloproteinases (MMPs). Research laboratories, pharmaceutical R&D departments, and diagnostic kit manufacturers need this high-purity biochemical for developing sensitive and reliable enzymatic assays.

Application

• Protease Substrate: Acts as a chromogenic or colorimetric substrate for the study of collagenase, gelatinase, and related matrix metalloproteinase (MMP) activities.
• Enzyme Kinetics Research: Used in biochemical assays to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) for target proteases.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Diagnostic Development: Serves as a critical component in the development of in vitro diagnostic (IVD) tests for diseases involving abnormal protease activity.
• Biochemical Tool Compound: Utilized in academic and industrial research to investigate protein degradation pathways and cellular remodeling processes.
• Quality Control Reagent: Applied as a standard or reference material in the quality control of enzyme preparations and related biopharmaceutical products.

Basic Information

Product Name	4-Phenylazobenzyloxycarbonyl-L-Pro-Leu-Gly-Pro-D-Arg Dihydrate
CAS No.	17011-78-8
Molecular Formula	C40H56N10O9 · 2H2O
Molecular Weight	856.97 g/mol (Anhydrous basis: 820.94 g/mol)
Synonyms	Pz-Pro-Leu-Gly-Pro-D-Arg · 2H2O; 4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg Dihydrate; Pz-PLGPR; Chromogenic Substrate Pz-PLGPR; MMP Substrate Pz-PLGPR; Collagenase Substrate; (4-Phenylazophenyl)methoxycarbonyl-L-prolyl-L-leucyl-glycyl-L-prolyl-D-arginine Dihydrate; PAB-Pro-Leu-Gly-Pro-D-Arg Dihydrate
EINECS	Contact for details

Quality Control

Our 4-Phenylazobenzyloxycarbonyl-L-Pro-Leu-Gly-Pro-D-Arg Dihydrate is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification, to meet stringent specifications for biochemical use. A detailed Certificate of Analysis (COA) documenting identity, purity, water content, and specific activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	3.5 - 5.5%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Biological Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.