Description

Ellman Ligand CAS NO 17005-57-1 is a specialized organosulfur compound widely recognized for its role as a chromogenic thiol detection reagent. This chemical is essential for quantifying sulfhydryl groups in peptides, proteins, and other biological molecules with high sensitivity and specificity. It is a critical tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors.

Application

• Thiol Group Quantification: Primary application for determining free sulfhydryl (-SH) content in proteins, peptides, and enzymes.
• Bioconjugation Monitoring: Used to track and quantify thiol-maleimide or thiol-haloacetyl conjugation reactions in antibody-drug conjugate (ADC) development and protein labeling.
• Pharmaceutical Analysis: Employed in the quality control of biopharmaceuticals to assess protein structure, folding, and stability by measuring exposed cysteine residues.
• Biochemical Research: A fundamental reagent in studying redox states, oxidative stress, and the activity of thiol-dependent enzymes like caspases and proteases.
• Diagnostic Kit Component: Incorporated into commercial assay kits for the colorimetric detection of glutathione, cysteine, and other biological thiols.
• Material Science: Used in surface chemistry to functionalize materials with thiol groups for sensor development and nanotechnology applications.

Basic Information

Product Name	Ellman Ligand CAS NO 17005-57-1
CAS No.	17005-57-1
Molecular Formula	C14H8N2O8S2
Molecular Weight	396.35 g/mol
Synonyms	5,5'-Dithiobis(2-nitrobenzoic acid); DTNB; Ellman's Reagent; 3-Carboxy-4-nitrophenyl disulfide; Bis(3-carboxy-4-nitrophenyl) disulfide; 4,4'-Dithiobis(3-nitrobenzoic acid); Thionitrobenzoic acid; DTNBA
EINECS	241-052-0

Quality Control

Our Ellman Ligand is produced under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research and analytical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant industry standards to support our customers in pharmaceutical development and high-precision biochemical research.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be kept in a desiccated environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to yellow-green crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Extinction Coefficient	13,600 - 14,200 M-1cm-1 at 412 nm
Solubility (Buffer, pH 8.0)	Clear, yellow solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.