Description

5-Bromo-3-Indolyl-β-D-Glucopyranoside is a chromogenic substrate widely used in enzymatic assays and molecular biology research. This compound is critical for the detection and quantification of β-glucosidase activity, serving as a key reagent in diagnostic kits and research protocols. It is primarily needed by biotechnology firms, diagnostic manufacturers, and academic research laboratories focused on enzyme kinetics, cell biology, and genetic screening.

Application

• β-Glucosidase Detection: As a chromogenic substrate for the specific detection and histochemical localization of β-glucosidase activity in biological samples.
• Molecular Biology Research: Used in cloning and gene expression studies, particularly in blue-white screening protocols with modified vectors.
• Diagnostic Kit Manufacturing: A key component in commercial diagnostic kits for enzymatic assays related to metabolic disorders.
• Microbiological Analysis: Employed in microbial identification and characterization based on specific glucosidase activity.
• Plant Biochemistry Studies: Used to study glucosidase enzymes involved in plant defense mechanisms and secondary metabolism.
• Quality Control in Biotechnology: Acts as a standard reagent for validating the activity of recombinant β-glucosidase enzymes.

Basic Information

Product Name	5-Bromo-3-Indolyl-β-D-Glucopyranoside
CAS No.	16934-09-1
Molecular Formula	C14H16BrNO6
Molecular Weight	374.18 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside; X-Gluc; 5-Bromo-3-indolyl β-D-glucoside; 5-Br-3-indoxyl-β-D-glucopyranoside; 5-Bromo-4-chloro-3-indolyl-β-D-glucoside; β-Glucuronidase substrate (X-Gluc); BCI-glucoside; 16934-09-1
EINECS	Contact for details

Quality Control

Our 5-Bromo-3-Indolyl-β-D-Glucopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant quality management standards to support our global B2B clients in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	< 10 ppm
Performance (Enzymatic Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.