Description

4-Nitrophenyl2-o-(B-D-Glucopyranosyl)-B-D-Glucopyranoside is a synthetic chromogenic substrate specifically designed for enzymatic assays. This compound matters because it provides a reliable and sensitive method for detecting and quantifying the activity of α-glucosidase and related hydrolytic enzymes. Industries and research institutions involved in biochemistry, pharmaceutical development, and diagnostic reagent manufacturing need this high-purity substrate for critical quality control and research applications.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of α-glucosidase activity.
• Biochemical Research: Used in kinetic studies and inhibitor screening for enzymes involved in carbohydrate metabolism.
• Diagnostic Reagents: A key component in clinical chemistry kits for the diagnosis of metabolic disorders like Pompe disease.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) to identify potential therapeutic agents targeting glycosidase enzymes.
• Quality Control: Essential for standardizing enzyme preparations and validating assay protocols in industrial biotechnology.
• Academic Research: Fundamental tool for studying enzyme mechanisms and glycoside hydrolase family functions.

Basic Information

Product Name	4-Nitrophenyl2-o-(B-D-Glucopyranosyl)-B-D-Glucopyranoside
CAS No.	16790-33-3
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	4-Nitrophenyl α-D-glucopyranosyl-(1→2)-α-D-glucopyranoside; p-Nitrophenyl α-isomaltoside; α-PNPG2; 4-Nitrophenyl α-isomaltoside; PNPG2; p-Nitrophenyl-α-isomaltoside; α-D-Glucopyranosyl-(1→2)-α-D-glucopyranoside, 4-nitrophenyl; Substrate for α-glucosidase
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl2-o-(B-D-Glucopyranosyl)-B-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. We provide comprehensive analytical data, including HPLC purity, spectral identification (IR, NMR), and specific optical rotation. A Certificate of Analysis (COA) detailing all specifications is available for every batch to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.