Description

Phenyl 1-Thio-β-D-Galactopyranoside is a specialized thioglycoside derivative of galactose, serving as a critical chromogenic and fluorogenic substrate for enzyme activity assays. Its core value lies in its selective hydrolysis by the enzyme β-galactosidase, producing a detectable color or fluorescence change that enables precise biochemical and molecular biology research. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, particularly for applications in gene expression studies, high-throughput screening, and microbial detection.

Application

• Substrate for β-Galactosidase Assays: Used as a chromogenic/fluorogenic reagent in enzymatic assays to measure β-galactosidase activity, a common reporter gene in molecular biology.
• Microbiological Research: Employed in media and tests for the identification and study of microorganisms, such as E. coli, that express the lacZ gene.
• High-Throughput Screening (HTS): A key component in drug discovery platforms for screening inhibitors or activators of β-galactosidase-linked pathways.
• Diagnostic Kit Manufacturing: Integrated into clinical and research diagnostic kits for detecting specific enzymatic activities associated with genetic disorders or cellular processes.
• Biochemical Research: Utilized in studying glycosidase mechanisms, enzyme kinetics, and carbohydrate-protein interactions.
• Cell-based Assays: Applied in live-cell imaging and flow cytometry to monitor gene expression and cellular events in real-time.

Basic Information

Product Name	Phenyl 1-Thio-β-D-Galactopyranoside
CAS No.	16758-34-2
Molecular Formula	C12H16O5S
Molecular Weight	272.32 g/mol
Synonyms	Phenyl 1-Thio-β-D-galactopyranoside; Phenyl Thiogalactoside; PTG; 1-Thio-β-D-galactopyranosyl phenyl; β-D-Galactopyranoside, phenyl 1-thio-; Phenyl-β-D-thiogalactopyranoside; Galactosyl phenyl sulfide; Phenyl β-D-thiogalactoside
EINECS	240-807-6

Quality Control

Our Phenyl 1-Thio-β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectroscopic identification, and residual solvent analysis, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and specific tests is provided with every shipment to guarantee traceability and consistency for our global B2B clients.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere or with desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	158 - 162 °C
Specific Rotation	-85° to -95° (c=1 in H2O)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.