Description

Acetylcholine-(Methyl-14C) Chloride is a radiolabeled derivative of the essential neurotransmitter acetylcholine, where the methyl group is tagged with the carbon-14 isotope. This product is critical for advanced research requiring precise metabolic and pharmacokinetic tracing in biological systems. It is primarily utilized by pharmaceutical R&D teams, neurobiology researchers, and contract research organizations (CROs) conducting autoradiography, ADME studies, and receptor binding assays.

Application

• Pharmacokinetic and ADME Studies: Tracing the absorption, distribution, metabolism, and excretion of acetylcholine and related cholinergic compounds in preclinical models.
• Neuropharmacology Research: Investigating cholinergic receptor binding, localization, and density using autoradiography and other radioligand binding techniques.
• Metabolic Pathway Analysis: Elucidating the metabolic fate and enzymatic pathways (e.g., via acetylcholinesterase) of acetylcholine in vitro and in vivo.
• Drug Discovery & Development: Serving as a key tracer in the development of new therapeutics targeting the cholinergic system for conditions like Alzheimer's disease and myasthenia gravis.
• Biochemical Assay Development: Use as a standard or tracer in developing high-throughput screening assays for enzyme activity or inhibitor discovery.

Basic Information

Product Name	Acetylcholine-(Methyl-14C) Chloride
CAS No.	16597-28-7
Molecular Formula	C714CH16ClNO2 *
Molecular Weight	~182.66 g/mol (unlabeled) *
Synonyms	(2-Acetoxyethyl)trimethylammonium-(methyl-14C) Chloride; [Methyl-14C]Acetylcholine Chloride; ACh-14C; 14C-Acetylcholine Chloride; (2-Acetyloxyethyl)trimethylazanium-(methyl-14C) Chloride; 14C-ACh; Radiolabeled Acetylcholine Chloride
EINECS	Contact for details

* Formula and weight are for the unlabeled compound. Specific activity and exact molecular weight for the radiolabeled product are provided on the Certificate of Analysis.

Quality Control

Every batch of Acetylcholine-(Methyl-14C) Chloride is manufactured and analyzed under strict quality control protocols to ensure identity, purity, and specific activity. Comprehensive analysis includes radiochemical purity determination by HPLC and TLC, specific activity measurement, and confirmation of chemical and isotopic identity. A detailed Certificate of Analysis (COA) is supplied with each shipment, providing full traceability and compliance with relevant radiochemical handling standards.

Storage

Preserve in the original container as supplied, tightly sealed, and stored at or below -20°C. This product is both light-sensitive (store away from light) and hygroscopic (moisture-sensitive). For long-term stability, store in a dedicated freezer, protected from light and moisture. Allow the vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow solution
Identification (HPLC, TLC)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	50-60 mCi/mmol (1.85-2.22 GBq/mmol)
Chemical Purity (HPLC)	≥ 95%
Solvent	Ethanol: Water solution
Concentration	1 mCi/mL (37 MBq/mL)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.