Description

4-(3-Methyl-3H-Diazirin-3-Yl)Butanoic Acid is a specialized diazirine-based carboxylic acid derivative, serving as a critical photoaffinity labeling reagent and crosslinker in advanced biochemical research. Its core value lies in its ability to form highly reactive carbene intermediates upon photolysis, enabling the covalent tagging and study of biomolecular interactions. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and chemical biology who require precise tools for target identification and mechanism-of-action studies.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalently attaching probes to biological targets (e.g., proteins, nucleic acids) upon UV light activation for studying drug-target interactions.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to identify and characterize enzyme active sites and binding pockets in complex biological samples.
• Bioconjugation & Crosslinking: Facilitates the creation of stable crosslinks between biomolecules or between a biomolecule and a solid support for analytical or diagnostic applications.
• Pharmaceutical Research: Integral in early-stage drug discovery for target validation and mapping small molecule binding sites.
• Material Science: Employed in surface functionalization and the development of smart polymeric materials where light-triggered crosslinking is required.
• Synthesis of Advanced Probes: Serves as a versatile building block for synthesizing more complex photoactivatable probes, tags, and linkers.

Basic Information

Product Name	4-(3-Methyl-3H-Diazirin-3-Yl)Butanoic Acid
CAS No.	16297-97-5
Molecular Formula	C7H10N2O2
Molecular Weight	154.17 g/mol
Synonyms	3-[3-(Carboxypropyl)]-3-methyldiazirine; 4-(3-Methyldiazirin-3-yl)butyric Acid; 3-Methyl-3-(3-carboxypropyl)diazirine; Diazirine Butanoic Acid Derivative; Photoactivatable Butanoic Acid; PAL Reagent; Carbene Precursor Acid; (3-Methyl-3H-diazirin-3-yl)butanoic acid
EINECS	Contact for details

Quality Control

Every batch of 4-(3-Methyl-3H-Diazirin-3-Yl)Butanoic Acid is manufactured and tested under strict quality management protocols to ensure high purity and consistent performance for sensitive research applications. We provide comprehensive analytical documentation, including Certificates of Analysis (COA) with detailed HPLC, NMR, and MS data. Our quality systems are designed to support GMP/GLP-compliant research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and degradation. For long-term stability, desiccate if necessary. Keep away from heat and incompatible materials.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.