Description

L-Dithiothreitol (DTT) is a powerful, water-soluble reducing agent widely used to cleave disulfide bonds within and between biological molecules. Its primary value lies in its ability to maintain proteins and enzymes in a reduced, active state, which is critical for numerous biochemical and biotechnological processes. This high-purity reagent is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries, particularly for protein purification, sequencing, and cell culture applications.

Application

• Protein Biochemistry & Purification: Reduction of intra- and intermolecular disulfide bonds to denature proteins or maintain them in a reduced state during purification.
• Molecular Biology: Essential component in buffer systems for enzyme reactions (e.g., preventing oxidation of thiol groups in reverse transcriptase).
• Pharmaceutical Development: Used in the research and production of therapeutic proteins and antibodies to control folding and activity.
• Diagnostic Reagent Manufacturing: Stabilizing agent in diagnostic kits and buffers to ensure consistent assay performance.
• Cell Culture & Tissue Engineering: Application in media and buffers to create a reducing environment for specific cell types.
• Polymerase Chain Reaction (PCR): Often included in PCR buffers to enhance the stability and activity of DNA polymerase.
• Chromatography: Used in mobile phases for HPLC analysis of thiol-containing compounds to prevent oxidation.
• General Laboratory Reagent: A standard reducing agent for a wide range of chemical and biochemical experiments.

Basic Information

Product Name	L-Dithiothreitol
CAS No.	16096-97-2
Molecular Formula	C4H10O2S2
Molecular Weight	154.25 g/mol
Synonyms	DTT; Cleland's Reagent; (2S,3S)-1,4-Dimercaptobutane-2,3-diol; (S,S)-1,4-Dimercapto-2,3-butanediol; Dithiothreitol; L-DTT; Threo-1,4-dimercapto-2,3-butanediol; (R*,R*)-2,3-Dihydroxy-1,4-butanedithiol
EINECS	240-263-5

Quality Control

Our L-Dithiothreitol is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and production applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and specific impurity profiles. We can supply material that meets or exceeds specifications for various application standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) due to its properties of being easily oxidized and hygroscopic. For long-term storage, desiccate at -20°C. Allow the container to reach room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 99.0%
Optical Rotation [α]20/D	-19.0° to -21.0° (c=1 in water)
Melting Point	40 - 43 °C
Water Content (KF)	≤ 0.5 %
Residue on Ignition	≤ 0.1 %
Heavy Metals (as Pb)	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.