Description

L-Leucine p-Nitroanilide Hydrochloride is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of protease activity, particularly leucine aminopeptidase, due to its specific cleavage properties. It is an essential reagent for pharmaceutical R&D, diagnostic kit manufacturing, and academic research in enzymology and cell biology.

Application

• Enzymatic Substrate for Protease Assays: Primary substrate for the colorimetric and spectrophotometric determination of leucine aminopeptidase (LAP) activity.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize novel protease inhibitors for drug discovery.
• Clinical Diagnostics: A key component in the formulation of diagnostic kits for measuring LAP levels in biological samples, aiding in liver and pancreatic function tests.
• Biochemical Research: Employed in studying enzyme kinetics, mechanism of action, and substrate specificity of various aminopeptidases.
• Quality Control in Biotechnology: Used to assess the purity and activity of enzyme preparations in biomanufacturing processes.

Basic Information

Product Name	L-Leucine p-Nitroanilide Hydrochloride
CAS No.	16010-98-3
Molecular Formula	C12H18N3O3Cl
Molecular Weight	287.75 g/mol
Synonyms	L-Leucine 4-nitroanilide hydrochloride; L-Leu-pNA HCl; 4-Nitroaniline, N-L-leucyl-, hydrochloride; L-Leucyl-4-nitroaniline hydrochloride; N-L-Leucyl-4-nitroaniline hydrochloride; Leucine p-nitroanilide hydrochloride; Substrate for Leucine Aminopeptidase
EINECS	240-163-6

Quality Control

Our L-Leucine p-Nitroanilide Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and specific tests for residual solvents and moisture. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent in-house specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant in the container.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.