Description

n-Benzoyl-Dl-Phenylalanine 2-Naphthyl Ester is a specialized synthetic chromogenic substrate designed for the determination and study of the proteolytic enzyme chymotrypsin. This compound is critical for biochemical research and quality control in enzymatic analysis, providing a reliable and sensitive means to measure chymotrypsin activity. It is primarily utilized by research institutions, pharmaceutical R&D laboratories, and diagnostic kit manufacturers involved in enzymology, protease research, and biochemical assay development.

Application

• Primary Substrate for Chymotrypsin Assays: Used as a specific chromogenic substrate in spectrophotometric and fluorometric assays to determine chymotrypsin concentration and activity.
• Enzyme Kinetics Research: Essential for studying the kinetic parameters (Km, Vmax) and inhibition mechanisms of chymotrypsin and related serine proteases.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits and reagents for clinical and research laboratories to detect and quantify chymotrypsin.
• Pharmaceutical Quality Control: Employed in the QC testing of pharmaceutical products where chymotrypsin activity is a critical parameter, such as in certain pancreatic enzyme supplements.
• Biochemical Research Tool: Used in academic and industrial research to investigate protein digestion, protease signaling pathways, and the development of new protease inhibitors.

Basic Information

Product Name	n-Benzoyl-Dl-Phenylalanine 2-Naphthyl Ester [For Determination Of Chymotrypsin]
CAS No.	15873-25-3
Molecular Formula	C26H21NO3
Molecular Weight	395.45 g/mol
Synonyms	Benzoyl-DL-phenylalanine 2-naphthyl ester; N-Benzoyl-DL-phenylalanine β-naphthyl ester; Bz-Phe-ONap; Chymotrypsin substrate; 2-Naphthyl N-benzoyl-DL-phenylalaninate; N-(Benzoyl)-DL-phenylalanine 2-naphthyl ester; Protease chromogenic substrate
EINECS	Contact for details

Quality Control

Our n-Benzoyl-Dl-Phenylalanine 2-Naphthyl Ester is produced and tested to meet the stringent requirements of research and diagnostic applications. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and functional activity testing against chymotrypsin. Certificates of Analysis (COA) detailing identity, purity, and performance specifications are provided with every shipment to ensure traceability and compliance with your quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Specification available upon request
Loss on Drying	≤ 0.5%
Functional Activity	Conforms to reference standard for chymotrypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.