Description

4-Maleimido-Tempo is a bifunctional spin-labeling reagent that combines a stable nitroxide radical (TEMPO) with a maleimide group. This compound is critical for site-directed spin labeling (SDSL) in electron paramagnetic resonance (EPR) spectroscopy, enabling the study of protein dynamics, structure, and conformational changes. It is primarily utilized by researchers and manufacturers in bioconjugation, structural biology, and advanced materials science for probing biomolecular interactions and developing functionalized polymers.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Covalently attaches to cysteine residues in proteins to study structure, dynamics, and folding.
• Bioconjugation and Protein Labeling: Used to create spin-labeled antibodies, enzymes, and other biomolecules for diagnostic and research applications.
• Polymer Functionalization: Incorporates stable radical sites into polymer backbones or surfaces for creating smart materials, sensors, or as polymerization regulators.
• Structural Biology Research: A key tool for investigating membrane protein topology, protein-protein interactions, and conformational changes in solution.
• Development of Nitroxide-Based Probes: Serves as a building block for synthesizing advanced molecular probes for imaging and redox studies.
• Crosslinking Agent for Specialty Polymers: Can be used to introduce crosslinks or specific functional groups into synthetic polymers.

Basic Information

Product Name	4-Maleimido-Tempo
CAS No.	15178-63-9
Molecular Formula	C13H19N2O4
Molecular Weight	261.30 g/mol
Synonyms	4-Maleimido-TEMPO; 4-Maleimido-2,2,6,6-tetramethylpiperidine-1-oxyl; Maleimido-TEMPO; 3-Maleimidopropyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; 4-Maleimido-2,2,6,6-tetramethylpiperidin-1-oxyl; 1-Oxyl-2,2,6,6-tetramethyl-4-piperidinyl maleimide; TEMPO maleimide; MTSSL (Maleimido-TEMPO Spin Label)
EINECS	Contact for details

Quality Control

Our 4-Maleimido-Tempo is produced under stringent quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and radical content. We adhere to ISO 9001 quality management principles, and our analytical methods include HPLC, EPR, and NMR to verify product integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. The container should be kept in a cool, dry, and well-ventilated area, separate from incompatible materials.

Specification

Item	Specification
Appearance	Orange to red powder or crystals
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
EPR Assay (Radical Content)	≥ 90%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.