Description

4-Nitrophenyl-n-Acetyl-β-D-Galactosaminide is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-galactosidase and related enzyme activities, providing a reliable colorimetric readout. It is primarily utilized by researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic industries for applications ranging from gene expression studies to microbial detection.

Application

• Enzymatic Substrate for β-Galactosidase: A key chromogenic reagent in biochemical assays for detecting β-galactosidase activity, commonly used in molecular biology (e.g., lacZ gene reporter assays).
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for clinical microbiology and pathology to identify specific bacterial enzymes.
• Pharmaceutical Research & Development: Employed in high-throughput screening (HTS) and drug discovery platforms to study enzyme inhibition or activation.
• Food & Beverage Quality Control: Applied in enzymatic methods for the detection of microbial contamination or specific enzymatic activity in products.
• Academic & Industrial Research: Serves as a fundamental tool in biochemistry and cell biology laboratories for studying glycosidase mechanisms and cellular processes.

Basic Information

Product Name	4-Nitrophenyl-n-Acetyl-β-D-Galactosaminide
CAS No.	14948-96-0
Molecular Formula	C14H17N3O8
Molecular Weight	355.30 g/mol
Synonyms	p-Nitrophenyl N-Acetyl-β-D-galactosaminide; 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-galactopyranoside; PNP-NAG; N-Acetyl-β-D-galactosaminide 4-nitrophenyl; 4-Nitrophenyl N-acetyl-β-D-galactosaminide; β-D-Galactosaminide, N-acetyl-, 4-nitrophenyl; GalNAc-β-pNP; 2-Acetamido-2-deoxy-β-D-galactopyranosyl 4-nitrophenyl
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-n-Acetyl-β-D-Galactosaminide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere or with desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.