Description

Phenolphthalein Monophosphate Dicyclohexylammonium Salt is a high-purity chromogenic substrate specifically designed for enzymatic detection and quantification. This compound is critical for research and diagnostic applications requiring precise and sensitive measurement of alkaline phosphatase activity. It is primarily utilized by manufacturers and researchers in the life sciences, clinical diagnostics, and biotechnology sectors for developing assays and diagnostic kits.

Application

• Primary Substrate for Alkaline Phosphatase (ALP): Used as a key reagent in enzymatic assays for the detection and quantification of ALP activity in biochemical research and clinical diagnostics.
• ELISA and Immunoassays: Serves as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other immunoassay formats, producing a measurable color change upon enzymatic cleavage.
• Diagnostic Kit Manufacturing: An essential raw material for the production of commercial diagnostic kits used in clinical laboratories for liver function tests, bone disease markers, and other ALP-related pathologies.
• Histochemistry and Cell Staining: Employed in laboratory techniques for the localization of alkaline phosphatase activity in tissue sections and cell cultures.
• Molecular Biology Research: Used in various research applications, including reporter gene assays and blotting techniques (e.g., Western, Southern) that utilize alkaline phosphatase conjugates.
• Quality Control in Biotechnology: Applied in the quality control processes of biopharmaceutical production to monitor enzyme activity and process consistency.

Basic Information

Product Name	Phenolphthalein Monophosphate Dicyclohexylammonium Salt
CAS No.	14815-59-9
Molecular Formula	C32H43N2O8P
Molecular Weight	614.67 g/mol
Synonyms	Phenolphthalein Diphosphate Dicyclohexylammonium Salt; Phenolphthalein Phosphate, Dicyclohexylammonium Salt; Dicyclohexylammonium Phenolphthalein Monophosphate; ALP Substrate; Alkaline Phosphatase Substrate; PPP; Phenolphthalein-3',3''-bis(phosphate) Dicyclohexylammonium Salt; 3,3-Bis(4-hydroxy-3-oxo-1(3H)-isobenzofuranylidene)-1(3H)-isobenzofuranone Monophosphate Dicyclohexylammonium Salt
EINECS	Contact for details

Quality Control

Our Phenolphthalein Monophosphate Dicyclohexylammonium Salt is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO; slightly soluble in ethanol
Functional Performance (ALP Assay)	Conforms to reference activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.